一种改良的吻合法小鼠腹腔异位心脏移植模型  

A Modified Mouse Abdominal Heterotopic Heart Transplantation Model by Anastomosis Technique

作　　者：姜晓峰[1] 朱磊[1] 崔哲铭[1] 孙文郁[1] 郭大伟[1] 林琳[1] 唐裕福[1] 王学范[1] 梁健[1] 

机构地区：[1]中国医科大学附属第四医院外科,沈阳110032

出　　处：《中国普外基础与临床杂志》2010年第9期914-916,共3页Chinese Journal of Bases and Clinics In General Surgery

基　　金：教育部留学回国人员科研启动基金资助项目(项目编号:2008-890);中国医科大学附属第四医院院内课题项目(项目编号:2006J06)~~

摘　　要：目的应用一种新的吻合法建立小鼠腹腔异位心脏移植模型,提高移植手术的成功率及移植物存活率,减少手术并发症。方法供体心脏异位移植于受体腹腔,将供体心脏升主动脉和肺动脉分别与受体腹主动脉和肝下下腔静脉吻合。不单独对供体肺门组织进行结扎,也不结扎受体腰静脉,不将受体腹主动脉与下腔静脉分离,而是一并游离并阻断。结果本研究进行手术229例,手术成功率为97.82%(224/229)。同种同系移植物存活时间均在6个月以上。并发症发生情况:主动脉血栓2例(0.87%),下腔静脉血栓1例(0.44%),心脏扭转4例(1.75%),出血4例(1.75%),下肢麻痹2例(0.87%),肠梗阻1例(0.44%),无麻醉意外发生。结论改良的小鼠腹腔异位心脏移植术简单可靠,较其他方法节省了手术时间并减少了出血的可能,为移植免疫学研究提供了一种实用的方法。Objective To establish a modified mouse abdominal heterotopic heart transplantation model in order to increase the graft survival rate and reduce operative complications. Methods The heart was transplanted into the abdomen by anastomosing the donor ascending aorta and pulmonary artery to the recipient abdominal aorta and infrahepatic vena cava respectively. Hilar tissue was not alone ligated,meanwhile recipient lumbar vein was not ligated. Recipient abdominal aorta and infrahepatic vena cava were not isolated,but were liberated and obstructed simultaneously. Results Two hundred and twenty-nine formal transplantations were performed with the successful rate of 97.82% （224/229）. The syngeneic graft survival time was more than 6 months. Complications：Aorta thrombus was found in 2 mice （0.87%）,inferior vena cava thrombus in 1 mouse （0.44%）,heart torsion in 4 mice （1.75%）,hemorrhage in 4 mice （1.75%）,crural paralysis in 2 mice （0.87%）,intestinal obstruction in 1 mouse （0.44%）,and no anesthetic accident happened. Conclusions The meliorated mouse abdominal heterotopic heart transplantation model is simple and reliable,which can reduce the operation time. Thus,the meliorated method provides a useful technique for immunologic transplantation research.

关 键 词：心脏移植 小鼠 吻合技术 

分 类 号：R654.2[医药卫生—外科学]