CD1a^+树突状细胞在胎儿腭扁桃体内的发育  

Development of CD1a^+DC in human fetal platine tonsil

作　　者：胡蓉[1] 苏敏[1] 黄悦[1] 李红[1] 姜俸蓉[1] 许庭良[1] 

机构地区：[1]贵阳医学院组织学与胚胎学教研室,贵阳550004

出　　处：《第三军医大学学报》2010年第17期1854-1856,共3页Journal of Third Military Medical University

基　　金：贵州省教育厅基金(2002116)~~

摘　　要：目的探讨CD1a+树突状细胞(dendritic cell,DC)在胎儿腭扁桃体内的发育。方法收集9～28周因故终止妊娠胎儿腭扁桃体标本29例,用EnVision二步法染色显示CD1a+DC。结果 12周胎儿腭扁桃体,可见少量CD1a+DC,分布于隐窝上皮基底层和中间层,细胞体梭形或不规则形,由细胞体发出数根树枝样突起,细胞核圆形或卵圆形。14周胎儿腭扁桃体,CD1a+DC数量增加,隐窝上皮周围的固有层弥散淋巴组织内也可见CD1a+DC。16周胎儿腭扁桃体,表面上皮内可见CD1a+DC分布。17～24周胎儿腭扁桃体,随着CD1a+DC数量增多,在上皮浅层渐可见阳性细胞。28周胎儿腭扁桃体,淋巴小结内始见几个CD1a+DC聚集。细胞计数显示CD1a+DC数,9～12周胎儿腭扁桃体为(2.60±1.14)个,随胎龄增加至25～28周胎儿腭扁桃体达(57.20±6.06)个,任意胎龄组间比较具有显著性差异(P<0.05)。结论 CD1a+DC12周迁入胎儿腭扁桃体隐窝上皮,随后逐渐出现在固有层弥散淋巴组织、表面上皮以及淋巴小结内。CD1a+DC数量随胎龄增长逐渐增多,主要分布于隐窝上皮内,固有层弥散淋巴组织和表面上皮内较少,淋巴小结内最少。Objective To study the development of CD1a＋DC in human fetal platine tonsil. Methods Twenty-nine cases of human fetal palatine tonsil were collected at gestational weeks 9-28. CD1a＋DC were identified with EnVision double staining. Results A small number of CD1a＋DC were detected at gestational week 12,which were distributed in basal lamina and stratum intermedium of crypt epithelium in human fetal platine tonsil. The cell bodies were fusiform or irregular in shape with several tree branch-like eruptions derived from them. The cell nuclei were round or oval in shape. The number of CD1a＋DC was increased at gestational week 14 with CD1a＋DC observed in diffused lymphoid tissue around the crypt. CD1a＋DC were distributed in surface epithelium at gestational week 16. Positive CD1a＋DC were gradually observed in lamina superficialis of endepidermis due to the increased number of CD1a＋DC from gestational weeks 17-24. Several CD1a＋DC began to emerge in lymphoid nodules at gestational week 28. The number of CD1a＋DC was increased from 2.60±1.14 at gestational weeks 9-12 to 57.20±6.06 at gestational weeks 25-28 with the growth of fetal age （P0.05）. Conclusion CD1a＋DC are immigrated into the crypt epithelium of human fetal palatine tonsil at gestational week 12,and can be gradually observed in diffused lymphoid tissue,surface epithelium and lymphoid nodules. The number of CD1a＋DC increases gradually with the growth of fetal age. CD1a＋DC are mainly distributed in crypt epithelium,less in diffused lymphoid tissue and surface epithelium,and least in lymphoid nodules.

关 键 词：CD1A DC 免疫组织化学 腭扁桃体 胎儿 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学] R392.12[医药卫生—基础医学]