壳聚糖修饰的羟基磷灰石-磷酸三钙修复犬牙槽骨缺损的实验研究  

Experimental Study of HA-TCP that Modified with CS on Repairing Bone Defect

作　　者：姜凌云[1] 王爽[1] 李艳萍[1] 牛玉梅[1] 

机构地区：[1]哈尔滨医科大学口腔医学院牙体牙髓病科,黑龙江哈尔滨150001

出　　处：《口腔医学研究》2010年第4期479-481,共3页Journal of Oral Science Research

基　　金：黑龙江省卫生厅项目(编号:2006-485);黑龙江省教育厅项目(编号:11521109)

摘　　要：目的:研究经壳聚糖(Chitosan,CS)修饰的羟基磷灰石-磷酸三钙(hydroxyapatite-tricalcium,HA-TCP)复合材料修复犬牙槽骨缺损。方法:制备CS溶液修饰HA-TCP。以CS修饰的HA-TCP复合材料修复犬牙槽骨缺损,选取3只成年犬876︱678作为受试牙,在受试牙根分歧处制备长宽高均为5mm的箱型骨缺损,分成两组,左侧作为实验组:将CS修饰的HA-TCP复合材料植入犬牙槽骨缺损部位;右侧作为对照组:将HA-TCP植入犬牙槽骨缺损部位;植入8周后将实验动物处死,组织学观察并图像分析新生骨组织的形成状态。结果:实验组新生骨组织占缺损的百分比明显高于对照组,有统计学意义(P<0.01);实验组剩余材料占缺损的百分比小于对照组,有统计学意义(P<0.01)。结论:CS修饰后的HA-TCP复合材料修复犬牙槽骨缺损效果优于单纯HA-TCP修复牙槽骨缺损。Objective：To study the efficacy of using HA-TCP（hydroxyapatite-tricalcium）modified with CS（Chitosan）for repairing alveolar bone defect.Methods：We modified the HA-TCP with the solution of CS and then,repaired bone defect with HA-TCP-CS composite.Defect samples that were 5mm×5mm×5mm at root furcation of 876︱678from three adult dogs were made.Test samples were divided into 2groups.All defects at the left of mandibles implanted with HA-TCP-CS composites were used as experimental group,and all defects at the right implanted with HA-TCP were used as control group.The animals were put to death after 8weeks.Histological oberservation and image measurement were performed on implantation section,and then analysied the condition of new bone formation of experimental and control groups.Results：Compared with the control group,there was more newly formed bone tissue,and less implant material composite remained in the experimental group.The difference was statistically significant（P0.01）.Conclusion：HA-TCP modified with CS deployed to repair alveolar bone defects in dog is better than HA-TCP only.

关 键 词：壳聚糖(CS) 羟基磷灰石-磷酸三钙(HA-TCP) 牙槽骨缺损 

分 类 号：R318.08[医药卫生—生物医学工程]