过氧化物酶体增殖物激活受体γ在大鼠脊髓损伤后继发性损伤中的表达变化  

Expression changes of peroxisome proliferator activated receptor γ following secondary injuries in rats with spinal cord injury

作　　者：王法臣[1] 袁绍纪[1] 冯华[2] 胡荣[2] 

机构地区：[1]济南军区总医院神经外科,济南250031 [2]第三军医大学西南医院神经外科,重庆400038

出　　处：《中华神经医学杂志》2010年第9期879-882,共4页Chinese Journal of Neuromedicine

摘　　要：目的 观察大鼠脊髓损伤后继发性损伤中过氧化物酶体增殖物激活受体γ（PPARγ）表达的变化.方法 将72只雄性SD大鼠按照随机数字表法分为损伤组（建立大鼠脊髓损伤模型）和对照组（仅显露脊髓,不做打击伤）,每组各36只.实时免疫荧光定量多聚酶链反应（FQ-PCR）检测脊髓损伤后1 d、3 d、7 d、14 d、28 d、56 dPPARγmRNA的表达变化,每个时间点取6只大鼠.蛋白印记检测脊髓损伤后1 d、3 d、7 d、14 d、28 d、56 d PPARγ的表达变化.结果 与对照组比较,损伤组脊髓组织中PPARγmRNA和PPARγ表达均明显增加,差异均有统计学意义（P＜0.05）,其表达高峰在脊髓损伤后14d.结论 脊髓损伤后在继发性损伤中PPARγ的表达明显增加,表达高峰在脊髓损伤后14d.Objective To observe the expression changes of peroxisome proliferator activated receptor γ（PPARγ） following the secondary injuries in rats with spinal cord injury （SCI）. Methods Seventy-two male SD rats were equally randomized into control group and SCI group. Real-time fluorescence quantitative polymerase chain reaction （FQ-PCR） was performed to detect the mRNA expression changes of PPARγ on the 1st, 3nd, 7th 14th, 28th and 56th d of SCI. Western blotting wasemployed to detect the protein expression changes of PPARγ Results Compared with those in the control group, the mRNA and protein expressions of PPARγ in the SCI group on the 1st, 3rd, 7th, 14th and 28th d of SCI were significantly increased （P〈0.05）, reaching its peak level 14 d after SCI; on the 56th d of SCI, their expression levels in the SCI group were still higher than those in the control group, but no significant differences were noted （P〉0.05）. Conclusion The expressions of PPARγare significantly increased following secondary injuries in rats with spinal cord injury, reaching its peak level 14 d after SCI.

关 键 词：过氧化物酶体增殖物激活受体 脊髓损伤 继发性损伤 

分 类 号：R651.2[医药卫生—外科学]