IFN-γ与IL-4基因多态性与儿童哮喘易感性及外周血IFN-γ、IL-4和IgE的关系  

Association of IFN-γ and IL-4 gene polymorphisms with childhood bronchial asthmatic susceptibility and the levels of plasma IFN-γ,IL-4 and IgE

作　　者：黄花荣[1] 吴敬芳[1] 

机构地区：[1]中山大学附属第二医院儿科,广东广州510120

出　　处：《中国病理生理杂志》2010年第9期1769-1775,共7页Chinese Journal of Pathophysiology

基　　金：广东省科技计划资助项目(No2009B030801082)

摘　　要：目的:探讨IFN-γ和IL-4基因多态性与儿童哮喘易感性及血浆IFN-γ、IL-4和IgE的相关性。方法:用聚合酶链反应-限制性酶切片段长度多态性(PCR-RFLP)法检测100例哮喘儿童和122例对照儿童IFN-γ基因-179G/T、IL-4基因-33C/T和-589C/T位点基因型;等位基因特异性-聚合酶链反应(AS-PCR)法检测IFN-γ基因+874A/T位点基因型;毛细管电泳法检测IFN-γ基因CA重复序列基因型;ELISA法测定血浆IFN-γ、IL-4和IgE。结果:100例哮喘儿童和122例对照儿童IFN-γ基因-179位点均为GG纯合子,未检测到突变基因型。IFN-γ基因+874A/T位点和CA重复序列的基因型和等位基因频率分布在哮喘组和对照组间无显著差异(P>0.05);+874位点多态性与血浆IFN-γ水平相关,AA基因型IFN-γ含量低于AT基因型(P<0.05)。IL-4基因-33C/T和-589C/T位点的基因型和等位基因频率分布在哮喘组和对照组间有显著差异(P<0.05);-33和-589位点TT基因型外周血IL-4和总IgE浓度均高于CT基因型,只有-33位点与血浆IL-4水平存在相关性(P<0.05)。结论:IFN-γ基因+874A/T和CA重复序列多态性可能与儿童哮喘无相关性,+874A/T位点多态性与IFN-γ水平相关。IL-4基因-33TT和-589TT基因型可能为儿童哮喘的易感基因型,-33位点多态性与IL-4表达水平相关。AIM： To investigate the gene polymorphisms of interferon-γ（ IFN-γ） and interleukin-4（ IL4） and the association with asthmatic susceptibility and the levels of plasma IFN-γ,IL-4 and IgE of asthmatic children. METHODS： 100 asthmatic children and 122 control children were enrolled the study. The genotypes of IFN-γ gene179G/T polymorphism,IL-4 gene-33C/T and-589C/T polymorphisms were tested by PCR-RFLP. The genotype of IFN-γ gene ＋ 874A/T polymorphism was tested by AS-PCR. The CA repeat polymorphism of IFN-γ gene was detected by capillary electrophoresis technique. The levels of serum IFN-γ,IL-4 and IgE were measured by ELISA. RESULTS： 100 asthmatic children and 122 control children were all GG homozygotes at-179 locus of IFN-γ gene.-179 locus of IFN-γ gene has no mutation. The genotypes and allele frequency of IFN-γ gene ＋ 874A/T and CA repeat polymorphisms showed no significant difference between asthmatic children and the control（ P 0. 05） . An association was revealed between IFN-γ gene ＋ 874A/T polymorphism and the level of plasma IFN-γ. The level of IFN-γ was lower in AA genotype than in AT genotype（ P 0. 05） . The genotypes and allele frequency of IL-4 gene-33C/T and-589C/T polymorphisms showed significant difference between asthmatic children and the control（ P 0. 05） . The levels of plasma IL-4 and IgE were higher in TT genotype at-33 locus and-589 locus than those in CT genotype,but only-33C/T polymorphism was associated with the level of plasma IL-4（ P 0. 05） . CONCLUSION： The IFN-γ gene ＋ 874A/T and CA repeat polymorphisms were not correlated with asthmatic susceptibility,but there is significant correlation between the level of IFN-γ and ＋ 874A/T polymorphism. TT genotype ofIL-4 gene-33 locus and-589 locus maybe the susceptible genotype of asthma in children,and the-33 locus polymorphism is associated with the level of IL-4.

关 键 词：干扰素-Γ 白细胞介素-4 基因多态性 哮喘 儿童 

分 类 号：R725.6[医药卫生—儿科]