硫化氢供体对急性支气管哮喘大鼠尾加压素Ⅱ表达的影响  

Effects of hydrogen sulfide on expression of urotensin II in acute asthmatic rats

作　　者：伍蕊[1] 陈亚红[1] 姚婉贞[1] 王配配[1] 耿彬[2] 唐朝枢[2] 

机构地区：[1]北京大学第三医院呼吸内科,北京100191 [2]北京大学医学部生理及病理生理系,北京100191

出　　处：《中国病理生理杂志》2010年第9期1781-1785,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No30871127);教育部新世纪人才支持计划资助项目(No985-2-082-113)

摘　　要：目的:探讨应用外源性硫氢化钠(NaHS,H2S供体)处理对卵白蛋白(OVA)诱导的大鼠急性支气管哮喘(简称哮喘)模型大鼠尾加压素Ⅱ(U-Ⅱ)表达的影响。方法:24只SPF级健康SD大鼠随机数字表法分为对照组、哮喘组和NaHS干预组,每组8只。致敏后28d测定所有大鼠肺功能。观察大鼠支气管周围病理炎症细胞浸润程度并进行评分;采用放射免疫法分别测定大鼠血浆、肺组织及肺泡灌洗液的U-Ⅱ含量。结果:(1)对照组、哮喘组和NaHS干预组大鼠最大呼气峰流速(PEF)分别为(6.5±0.1)L/s、(2.9±0.7)L/s及(5.7±0.5)L/s,3组间差异显著(F=112.129,P<0.01);(2)血浆U-Ⅱ含量分别为(45.3±18.1)ng/L、(61.5±27.2)ng/L、(47.3±11.8)ng/L,3组间无显著差异(F=1.546,P>0.05);(3)大鼠肺组织及肺泡灌洗液U-Ⅱ含量对照组分别为(16.6±3.4)ng/L及(26.3±7.8)ng/L,哮喘组分别为(43.8±2.0)ng/L及(58.0±12.3)ng/L,NaHS干预组分别为(14.0±1.9)ng/L及(20.2±6.7)ng/L,3组间差异显著(F=337.68、F=38.433,均P<0.01);(4)光镜下支气管周围炎症细胞浸润程度评分[用中位数(四分位数)表示],对照组为1(0-1)分,哮喘组为3(2-4)分,NaHS干预组1(1-2)分,3组间差异显著(H=16.925,P<0.01);(5)大鼠肺组织及肺泡灌洗液U-Ⅱ含量与光镜下支气管周围炎症细胞浸润程度评分均呈正相关(r=0.746、r=0.714,P<0.01),与PEF均呈负相关(r=-0.911、r=-0.767,均P<0.01)。结论:哮喘急性发作时气道U-Ⅱ分泌增加,U-Ⅱ可能以旁/自分泌的方式参与哮喘的发病;而H2S外源性供给可通过抑制U-Ⅱ合成/分泌,减轻哮喘急性发作时的气道炎症,对哮喘急性发病起到防治作用。AIM： To explore the effects of exogenously applied hydrogen sulfide （ H2S） on expression of urotensin II （ U-II） in rats with ovalbumin-induced acute asthma. METHODS： Twenty-four male SD rats were randomly divided into control group,asthma group and NaHS treatment group （ all n = 8） . At the 28th day after ovalbumin sensitization,the pulmonary function was measured. The pathological changes in the lung tissues were observed. The contents of U-II in plasma,lung tissues and bronchoalveolar lavage fluid （ BALF） was also detected. RESULTS： The peak expiratory flow （ PEF） was（ 6. 5 ± 0. 1） L/s,（ 2. 9 ± 0. 7） L/s and（ 5. 7 ± 0. 5） L/s in control group,asthma group and NaHS treatment group,respectively. No statistical difference of the plasma U-II levels was observed among the three groups. In asthma group,the content of U-II in lung tissues was（ 43. 8 ± 2. 0） ng/L and that in BALF was（ 58. 0 ± 12. 3） ng/L,both of which were significantly higher than those in control group [（ 16. 6 ± 3. 4） ng/L and（ 26. 3 ± 7. 8） ng/L]. In NaHS treatment group,the content of U-II in lung tissues was（ 14. 0 ± 1. 9） ng/L and that in BALF was（ 20. 2 ± 6. 7） ng/L,both of which were significantly lower than those in asthma group （ F =337. 68 and F =38. 433,respectiuely,both P 0. 01） . The pathological score of the lung tissues in asthma group was 3（ 2-4） ,significantly higher than that in control group[1（ 01） ]and NaHS treatment group[1（ 1-2） ]. The positive correlations between the contents of U-II in lung tissues or BALF and the pathological scores were observed （ r = 0. 746,r = 0. 714,respectively,both P 0. 01） . Significantly negative correlations between the contents of U-II in lung tissues or BALF and PEF were also found （ r =-0. 911 and r =-0. 767, respectively,both P 0. 01） . CONCLUSION： U-II,a mediator acting via the way of paracrine or autocrine,may play a role in the pathogenesis of asthma in this animal model. The exogenous applicatio

关 键 词：硫化氢 尾加压素Ⅱ 哮喘 大鼠 

分 类 号：R562.25[医药卫生—呼吸系统]