付里叶变换红外光谱法研究不同条件下菌紫质的结构  

FOURIER TRANSFORM INFRARED SPECTROSCOPIC STUDIES OF THE STRUCTURE BACTERIORHODOPSIN AT DIFFERENT HYDRATIONS

作　　者：张极震[1] 杨景文[1] 刘芳[1] 胡鑫尧[2] 孙素琴[2] 卢为琴[2] 

机构地区：[1]中国科学院生物物理研究所 [2]清华大学化学系

出　　处：《生物物理学报》1990年第2期147-152,共6页Acta Biophysica Sinica

摘　　要：本文用付里叶红外光谱法研究了干燥、水合及缓冲液内菌紫质的结构.酰胺Ⅰ和Ⅱ带有多个肩峰,在不同条件下,各峰的频率无明显位移.红外光谱表明,主要为α-螺旋结构,同时含有相当比例的反平行β-束及某些无规结构.酰胺Ⅰ带吸收频率最大值计算的α_n-螺旋频率.The structure of bacteriorhodopsin in purple membrane was investigated using fourier transform infrared spectroscoby. The samples were examined in buffer and after rehydrated over K2SO4 - saturated solution, air-and P2O5 -dried The spectra show that for all the four samples the amide I and II bands consist of a major absorption and several shoulders respectively, which suggests some proportion of β-strand, some are antiparallel in orientation, some β-turn and disordered structure besides a predominant α-helical structure. The amide I absorption frequency is dose to that calculated for αII - helical conformation. The hydrations do not change the presence of the amide I and n shoulders and their frequencies, indication that the hydrations do not affect the secondary structure of bacteriorhodopsin significantly.

关 键 词：菌紫质 结构 FTIR光谱 

分 类 号：Q71[生物学—分子生物学]