紫苏梗质量标准研究  

Study on Quality Specification of Caulis Perillae

作　　者：谷丽华[1,2] 郝希民[3] 赵森淼[1] 王峥涛[1,2] 

机构地区：[1]上海中医药大学中药研究所中药标准化教育部重点实验室,上海201210 [2]上海中药标准化研究中心,上海201210 [3]安徽中医学院药学院,合肥230031

出　　处：《中国药学杂志》2010年第17期1308-1312,共5页Chinese Pharmaceutical Journal

基　　金：<中国药典>2010年版一部标准研究课题资助(YS-179;YS-180);上海市重点学科建设资助项目(Y0301)

摘　　要：目的制定紫苏梗药材及饮片质量标准。方法采用了生药学研究,薄层鉴别法、HPLC、水分测定法及灰分测定法。薄层鉴别选用生物显色剂0.8 mg.mL-1的2,2-二苯基-1-苦肼基自由基(DPPH)乙醇溶液显色。结果根据10个不同批号的紫苏梗药材和5个不同批号的紫苏梗饮片测定结果,确定迷迭香酸含量不低于0.10%(药材)和0.07%(饮片),含水分不高于9.0%(药材)和8.0%(饮片),总灰分不高于5.0%(药材)和4.0%(饮片),酸不溶性灰分不高于1.2%(药材)和0.6%(饮片)。薄层色谱-生物自显影色谱图中迷迭香酸斑点清晰易辨。结论通过研究制定了紫苏梗的质量控制标准。OBJECTIVE To establish the quality standard for Caulis Perillae.METHODS The pharmacognostical methods were adopted to describe the characters of Caulis Perillae（prepared slice）.The bioactive constituents were analyzed by TLC and HPLC.TLC study was sprayed with 0.8 mg·mL^-1 2,2-diphenyl-1-picrylhydrazyl（DPPH）in ethanol.Moisture and ash were determined by the methods recorded in the Appendix of Chinese Pharmacopoeia（2005）.RESULTS The contents of total ash,acid-insoluble ash and moisture of 15 samples from different sources were determined.The moisture contents were below 9.0% and 8.0%（prepared slice）,respectively.The contents of the total ash were below 5.0% and 4.0%（prepared slice）,with the acid-insoluble content of 1.2% and 0.6%（prepared slice）,respectively.The contents of rosmarinic acid was above 0.10% and 0.07%（prepared slice）,respectively.TLC-bioautographic chromatograms were distinctly.CONCLUSION The established method can be used for the quality control of Caulis Perillae.

关 键 词：紫苏梗 质量标准 迷迭香酸 薄层色谱-生物自显影 

分 类 号：R917[医药卫生—药物分析学]