新型阳离子基因传递载体聚乙烯亚胺-醋酸酐的细胞转染研究  

Transfection with a Novel Cationic Gene Carrier: Polyethylenimine-Acetic Anhydride

作　　者：赵梦丹[1] 陈凤英[1] 虞和永[1] 

机构地区：[1]浙江大学医学院附属妇产科医院,杭州310006

出　　处：《中国药学杂志》2010年第18期1368-1371,共4页Chinese Pharmaceutical Journal

摘　　要：目的研究非病毒基因载体聚乙烯亚胺(PEI)-醋酸酐(ACA)共聚物的组成对体外介导基因传递的影响,评价其作为基因载体的可行性。方法通过粒径分析观察PEI-ACA与质粒DNA自组装形成的颗粒形态及粒径,预测其进入细胞的可能性。以MTT法检测PEI-ACA和PEI的细胞毒性。以PEI-ACA、PEI为载体,在不同的N/P比值的条件下,将绿色荧光蛋白基因pEGFP-C3导入真核细胞株A549细胞,以荧光显微镜及荧光分光光度计系统检测其转染效率。结果PEI-ACA转染效率高于PEI,同时PEI-ACA的细胞毒性显著低于PEI。结论新型阳离子多聚物PEI-ACA提高PEI介导的基因转染效率同时降低其细胞毒性,有望成为基因转染的有效载体。OBJECTIVE To study the influence of copolymer polyethylenimine（PEI）-acetic anhydride（ACA） composition on gene delivery in vitro and observe its potential as a gene carrier.METHODS Size measurement and AFM were used to show the modality of the PEI-ACA/DNA complexes.The cytotoxicity of PEI-ACA and PEI was evaluated by mononuclear cell direct cytotoxicity assay（MTT）.In different conditions,using PEI-ACA as the vector,A549 cells were transfected with fluorescent protein（pEGFP-C3） gene and 1ueiferase gene.Expression efficiency of the transduced gene was monitored by fluorescence microscopy and fluorescence assay system.RESULTS PEI-ACA had higher transfection efficiency with lower cytotoxicity than PEI.CONCLUSION PEI-ACA copolymer reduces the cytotoxicity of PEI,improves the transfection efficiency,and offers greater biocompatibility than PEI.It shows considerable potential as an efficient non-viral carrier for gene delivery.

关 键 词：非病毒载体 多聚乙烯亚胺 醋酸酐 基因转染 细胞毒性 

分 类 号：Q503[生物学—生物化学]