角果木提取物Tagalsin对H_(22)荷瘤小鼠Survivin和Caspase-3表达的影响  

The effect of ceriops tagal extracts Tagalsin on Survivin and Caspase-3 expression of H_(22) tumor-bearing mice

作　　者：宋修岐[1] 邢智远[1] 郭云良[2] 姚如永[2] 

机构地区：[1]山东省青岛大学第二附属医院普外科,266042 [2]青岛大学第一附属医院

出　　处：《中国临床研究》2010年第9期748-751,共4页Chinese Journal of Clinical Research

基　　金：国家"863"项目(2006AA09Z446);青岛市科技局项目(07-2-1-8-NSH-1)

摘　　要：目的探讨角果木提取物Tagalsin对H22肝癌细胞的抑制作用及其机制。方法将H22肝癌细胞原位接种于小鼠肝脏,建立小鼠原位移植性肝癌模型,建立模型第10天将小鼠随机分为食用油空白对照组、卡莫氟阳性对照组和Tagalsin低、中、高剂量治疗组,末次给药24h后处死小鼠;观察各组荷瘤小鼠的生存状态及体重变化,并计算各组小鼠的肿瘤近似体积和脾脏指数;观察肿瘤组织病理学变化;免疫组化方法检测各组Caspase-3、Survivin蛋白的表达;逆转录-酶链聚合反应(RT-PCR)技术检测Caspase-3、Survivin基因的表达。结果 Tagalsin能有效抑制H22肝癌细胞的生长,高剂量可使荷瘤小鼠的脾脏指数降低(P<0.01)。Tagalsin低、中、高剂量治疗组的抑瘤率分别为17.89%、63.11%、71.78%,卡莫氟组的抑瘤率为63.12%;阳性对照组及Tagalsin中、高剂量治疗组Caspase-3蛋白的表达高于空白对照组(P均<0.05),而Survivin蛋白的表达低于空白对照组(P均<0.05);与空白对照组相比,Tagal-sin各治疗组及阳性对照组Caspase-3基因水平显著上调(P<0.05或P<0.01),而高剂量组及阳性对照组Survivin基因水平下调(P均<0.01)。病理变化:Tagalsin高剂量组及阳性对照组肿瘤细胞体积缩小,胞浆浓染,核固缩,核仁不清,肿瘤细胞可见破碎,有较多凋亡细胞分布。结论 Tagalsin对H22肝癌细胞具有明显抑制作用,其机制可能与上调Caspase-3基因表达而下调Survivin基因的表达有关。Objective To investigate the inhibitory effect of Tagalsin （ceriops tagal extracts） on H22 hepatoma cells.Methods Orthotopic transplantation tumor model of mouse was established by transplanting H22 mouse hepatoma cells to mouse liver,and ten days after the mice（n=60） were randomly divided into five groups：edible oil blank control group,carmofur（HCFU） positive control group and Tagalsin treatment group including low dose,middle dose and high dose subgroups（n=12 each）.All mice were killed 24 hours after last medication.Survival conditions,weight changes,calculated tumor similar volume,spleen index and tumor inhibition rate of tumor-bearing mice were compared,and pathological changes of tumor were observed.Apoptosis factors Caspase-3 and Survivin protein were detected by immunohistochemical method,and expression of Caspase-3 and Survivin genes were detected by reverse transcription polymerase chain reaction. Pathological specimen was observed under optical microscope by HE staining.Results Hepatoma growth can be effectively inhibited by Tagalsin.Spleen index of tumor-bearing mice was decreased in high dose Tagalsin （P〈0.01）.The tumor inhibition rates of low,middle and high dose group of Tagalsin were 17.89%,63.11% and 71.78% respectively,and the tumor inhibition rate of HCFU was 63.12%. Compared with blank control group,Caspase-3 protien expressions were significantly higher（all P〈0.05）,while Survivin protein expressions were significantly lower （all P〈0.05）in positive control group and Tagalsin treatment group of high and middle dose. Likewise,Caspase-3 gene expression were significantly enhanced （P〈0.05 or P〈0.01）in positive control group and Tagalsin treatment groups while Survivin gene expression were significantly dropped in positive control group and Tagalsin treatment group of high dose（all P〈0.01）than those in blank control group.Pathological changes showed that tumor cell volume was shrinked,and hyperchromatic cytoplasm,karyopyknosis,unclear nucleole,broken

关 键 词：Tagalsin 位肝癌移植瘤模型 免疫组化 逆转录-酶链聚合反应 CASPASE-3 SURVIVIN 

分 类 号：R651.15[医药卫生—外科学]