血管内皮生长因子在急性髓系白血病骨髓上清、血浆中的表达及意义  

Expression and its significance of vascular endothelial growth factor in bone marrow supernatants and plasma in patients with acute myeloid leukemia

作　　者：刘丽波[1] 宋艳秋[2] 徐公斌[1] 

机构地区：[1]吉林大学第四医院血液肿瘤科,长春市130011 [2]吉林大学第一医院血液肿瘤科

出　　处：《中国临床研究》2010年第9期752-753,共2页Chinese Journal of Clinical Research

摘　　要：目的探讨血管内皮生长因子(VEGF)在急性髓系白血病(AML)骨髓上清、血浆中的表达及意义。方法选取23例初诊未治的AML患者作为研究对象。抽取枸橼酸钠抗凝的外周血2ml、肝素抗凝的骨髓3～5ml,离心后分别抽取血浆、骨髓上清,贮存于-20℃冰箱待用。采用ELISA方法检测骨髓上清、血浆中VEGF的表达水平。骨髓上清VEGF对照选取8例非何杰金淋巴瘤无骨髓浸润患者,血浆VEGF对照选取5例健康体检者。结果初诊未治的AML患者骨髓上清、血浆VEGF的水平均明显高于对照组(P均<0.05);且骨髓上清水平明显高于血浆水平(P<0.05),二者呈正相关(r=0.484,P=0.031)。AML患者化疗后完全缓解组(n=6)与未缓解组(n=4)血浆VEGF水平均较治疗前明显升高(P均<0.05),但完全缓解组与未缓解组比较差异无统计学意义(P>0.05)。化疗后完全缓解组与未缓解组骨髓VEGF水平均较治疗前略有下降,但差异无统计学意义(P>0.05);未缓解组与缓解组比较差异亦不显著(P>0.05)。结论初诊未治的AML骨髓上清、血浆VEGF的表达水平均明显升高,且骨髓上清明显高于血浆,具有诊断价值,可能为AML的治疗提供新的途径,但化疗后VEGF的变化不能作为判断疗效的指标。Objective To determine the levels of vascular endothelial growth factor （VEGF） of bone marrow supernatants and plasma in patients with acute myeloid leukemia and assess its clinical implication.Methods Twenty-three patients with newly diagnosed acute myeloid leukemia were served as research objects. VEGF levels in bone marrow supernatants and plasma were measured by sandwich ELISA.The bone marrow supernatants in patients with non-Hodgkin＇s lymphoma of no bone marrow infiltration（n=8）and plasma of healthy subjects（n=5）were respectively served as controls.Results VEGF levels of pre-chemotherapeutic bone marrow supernatants and plasma in newly diagnosed patients with acute myeloid leukemia were significantly higher than those in control groups （all P〈0.05）,and VEGF levels of bone marrow supernatants were obviously higher than that of plasma（P〈0.05 ）.VEGF level in bone marrow supernatants was correlated with VEGF level in plasma（r=0.484,P=0.031）.The plasma VEGF levels either in complete remission group（n=6） or in non-remission group（n=4） after therapy were all obviously higher than those before chemotherapy（all P〈0.05）,but there was no significance in two groups（P〉0.05）.VEGF levels of bone marrow supernatants in complete remission group and non-remission group after therapy were all decreased than those before chemotherapy,but had no statistical significance（P〉0.05）,and there was no significance in the two groups（P〉0.05）.Conclusions The VEGF levels of pre-chemotherapeutic bone marrow supernatants and plasma in newly diagnosed patients with acute myeloid leukemia are significantly increased,and level of bone marrow supernatants is markedly higher than that of plasma.There are changes after chemotherapy in the levels.The results have diagnostic value about acute myeloid leukemia,and might provide a new way for the treatment of acute myeloid leukemia,but could not be an index to assess therapeutic effect.

关 键 词：血管内皮生长因子 急性髓系白血病 骨髓上清 血浆 

分 类 号：R557[医药卫生—血液循环系统疾病]