ET-1和TGF-β_1在自发性高血压大鼠早期肾损害作用的实验研究  

Study of ET-1 and TGF-β_1 in the Early Renal Damage in Spontaneously Hypertensive Rats

作　　者：朱萱萱[1] 严冬[1] 达庆国[1] 严士海[1] 吴旭彤[1] 符蕾[1] 秦晓康[1] 

机构地区：[1]南京中医药大学附属医院,南京210029

出　　处：《中国医药导刊》2010年第8期1393-1394,共2页Chinese Journal of Medicinal Guide

基　　金：江苏省高校自然科学研究项目(08KGD360004)

摘　　要：目的:探讨自发性高血压(SHR)大鼠肾脏功能与ET-1和TGF-β_1的关系。方法:采用SHR大鼠和Wistar大鼠,每周用无创套尾法测量清醒状态下SHR大鼠和Wistar大鼠的收缩压、舒张压和心律。第8周和12周后收集尿液和取血,测定尿微量白蛋白、微量蛋白/肌酐、尿NAG和肌酐含量,用ELISA法分别测定大鼠血清中ET-1和TGF-β_1含量。结果:两组血清ET-1和TGF-β_1含量具有显著性差异(P<0.01);两组尿微量白蛋白、微量蛋白/肌酐、尿NAG和肌酐含量具有显著性差异(P<0.01)。结论:SHR大鼠血压增高与血清中ET-1和TGF-β_1浓度有关;尿微量白蛋白、微量蛋白/肌酐、尿NAG和肌酐含量的增高可能是引起高血压早期肾脏功能损害的重要因素。Objective： To investigated the spontaneously hypertensive （SHR） rats renal function relation with ET-1 and TGF-β1. Methods：SHR rats and Wistar rats, each week used non-invasive slip tail to measure systolic blood pressure, diastolic blood pressure and heart rate of awake SHR rats and Wistar rats. After the first 8 weeks and 12 weeks conected urine and blood, measured urine albumin, trace protein/creatinine, urine NAG, and creatinine concentration. By ELISA measured ET-I and TGF-β1 levels in serum. Results：ET-I and TGF-β1 levels in seram were significant differences （P〈0.01）; two groups of urinary albumin, trace protein/creafinine, urine NAG, and ereatinine levels were significant differences （P〈0.01）. Conclusions： SHR rat blood pressure up and related with serum ET-1 and TGF-β1 concentration; It was an important factor of hypertensive early renal dysftmcfion may be caused by urinary albumin, trace protein/creatinine, urinary NAG and creatinine levels up.

关 键 词：SHR和Wistar大鼠 ET-1和TGF-β1 肾脏功能 

分 类 号：R544.1[医药卫生—心血管疾病]