反相高效液相色谱法测定盐酸安非他酮血药浓度  

The Determination of Serum Bupropion Hydrochloride Concentrations in Patients by RP-HPLC

作　　者：张国阳[1] 李坚[1] 陈桂兵[1] 

机构地区：[1]淮安市第三人民医院,淮安223001

出　　处：《中国医药导刊》2010年第8期1427-1428,共2页Chinese Journal of Medicinal Guide

摘　　要：目的：建立盐酸安非他酮血药浓度测定方法.方法：反相高效液相色谱法,采用色谱柱：Kromasil C18（5μm,4.6mm×250mm） 流动相：甲醇：40mmol/L NH4AC[（乙酸调pH（4.5±0.1）]：（60：47,v/v） 流量：1.0ml/min 柱温：室温 检测波长：254nm 进样量：20μl.内标物：盐酸普萘洛尔.结果：以盐酸安非他酮与内标物峰面积之比（y）对盐酸安非他酮血清浓度（C）进行线性回归,得回归方程：C=1.13×102y-1.12×10,（r=0.9995,n=7）,结果表明：盐酸安非他酮在5.0～500.0μg/L范围内线性关系良好.结论：本方法操作简便、回收率高、精密度好.可以用于盐酸安非他酮的治疗药物监测.Objective：To develop an HPLC method for the quantitative determination of Bupropion Hydrochloride in patient＇s serum. Methods： The residues were analyzed with an HPLC system Kromasil C18（5μm,4.6mm×250mm）with the mobile phase consisted of methanol： 40mmol/L NH4AC（With acetic acid adjust to pH 4.5±0.1）（60：47,v/v）,The flow rate was 1.0 ml/min and the detection wavelength was at 254nm and the Column temperature was room temperature. Propranolol hydrochloride was used as internal standard.Results：Linear regression analysis was made by Bupropion hydroehloride serum concentration（C） and the peak area ratio（y） of Bupropion hydrochloride vs. internal standard（Propranolol hydrochloride）,the regression equation was C=1.13×10^2y-1.12×10,（r =0.9995,n=7）. The results showed that： Bupropion Hydrochloride has a good linear relationship in the range of 5.0-500.0μg/L. Conclusions：The method provides a simple,high recovery and good precision way for monitoring serum level of Bupropion Hydrochloride, and this method can be used for therapeutic drug monitoring of Bupropion Hydrochloride.

关 键 词：盐酸安非他酮 反相高效液相色谱法 血药浓度 治疗药物监测 

分 类 号：R927.2[医药卫生—药学]