铝酞菁光敏反应中间产物的实验研究  

A STUDY ON INTERMEDIATES OF PHOTOSENSITIZATION OF SULFONATED ALUMINUM PHTHALOCYANINE

作　　者：陈暨耀[1] 陈士明[2] 陆亚蒙 陈开泰[1] 蔡怀新[1] 

机构地区：[1]复旦大学物理系 [2]复旦大学分析测试中心

出　　处：《生物物理学报》1990年第3期312-317,共6页Acta Biophysica Sinica

摘　　要：铝酞菁(AISPC)是有潜力用于光动力癌症疗法(PDT)的新光敏剂.本文应用ESR波谱技术,对AISPC光敏反应的中间产物进行实验研究,探讨了重水,β—胡萝卜素、迭氧钠、SOD、细胞色素C、甘露醇及乙醇等活性氧增进或抑制剂对光敏反应的影响.结果表明,(Al-SPC)在水溶液中的光敏反应,除之有~1O_2生成外,还有O_2^-及·OH自由基产生.但以~1O_2.中间产物为主.光敏疗法是一种有希望的肿瘤治疗新方法.但由于光敏剂血卟啉衍生物(HPD)组份复杂、红光吸收太弱等局限性,1985年以来,探寻结构明了、成份稳定、主吸收峰位于红光区(人体绍织透射的最佳波段)的新光敏剂的研究工作不断增多,其中尤以对酞菁类染料的研究较为集中.我组在研究出可溶于水的磺化铝酞菁后,亦已在离体人癌细胞及小鼠移植瘤实验中(待发表)获得杀伤癌细胞、癌组织的功效.随着酞菁染料作为新光敏剂的潜力被挖掘,其反应机制的研究也受到重视,但目前尚不甚明了,且较系统的研究也不多.本文应用ESR波谐技术,可2、2.6、6—四甲基—4羟基哌啶醇为探针,并利用~1O_2,0_2~1·OH消除剂,研究了磺化铝酞菁的光敏反应中间产物,探讨了其可能的反应机制.Sulfonated aluminum phthalocyanine (ALSPC), a new photosensitizer of pontential use in photodynamic cancer therapy, the intermediates of its photo-sensitization were studied in photochmical level. Using 2 ,2,6,6,-tetramethy 1-1 -piperidone (TMHP) as a probe, the nitroxyl free radical produced in ALSPC photochemical reaction was detected by ESR spectrum technic. The effects of enhancement and inhibition of D2O, NaN3 β-carotene, cytochrome C, SOD, mannitol and alcohol be tested, and the results shown that there were 102 Of and OH intermediates produced in ALSPC photochemical reaction but the O seemed the main product in process.

关 键 词：铝酞菁 光敏剂 光敏反应 

分 类 号：R912[医药卫生—药学]