细胞壁缺陷型淋病奈瑟菌cppB基因酶切图谱分析  

Analysis of the Cryptic Plasmid B Gene of Cell Wall Deficient Neisseria gonorrhoeae by Restriction Enzyme Map

作　　者：魏荣旋[1] 赵庆[1] 王和[2] 

机构地区：[1]遵义医学院附属三院检验中心,贵州遵义563002 [2]贵阳医学院微生物教研室,贵州贵阳550004

出　　处：《中国实验诊断学》2010年第9期1353-1355,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨细胞壁缺陷对淋病奈瑟菌隐蔽性质粒B(cppB)基因的影响以及细胞壁缺陷型淋病奈瑟菌cppB的变异特点。方法用青霉素诱导淋病奈瑟菌成为细胞壁缺陷型并获得其纯培养物,cppB基因特异性引物PCR检测细胞壁缺陷型纯培养物的cppB基因,并对其cppB基因PCR扩增产物进行限制性核酸内切酶图谱分析。结果细菌型和细胞壁缺陷型均具有cppB基因扩增产物,经过限制性内切酶HindⅢ、HinfⅠ、HpaⅡ和MspⅠ消化和电泳,在5%PAGE凝胶电泳中,呈现出相同的电泳条带。结论淋病奈瑟菌细菌型及其细胞壁缺陷型对cppB基因限制性核酸内切酶(HindⅢ、HinfⅠ、MspⅠ、HpaⅡ)的分析没有发现淋病奈瑟菌L型具有与其亲代细菌型不同的图谱,提示细胞壁缺陷没有导致淋病奈瑟菌的cppB基因发生这些核酸酶切位点中核苷酸序列的改变。Objective To probe influence of cell wall deficiency on the Cryptic Plasmid B gene of N.gonorrhoeae and its characteristic variation.Methods The N.gonorrhoeae was induced into L-form by penicillin and the cppB gene of the pure culture of L-form was determined by PCR and Restriction Enzyme（HindⅢ,HinfⅠ,MspⅠ,HpaⅡ）.Results By PCR,the cppB genes were be found in both the bacterial form and the L-forms of N.gonorrhoeae.There were same bands in the bacterial form and the L-forms by Restriction Enzyme Map.Conclusion The Restriction Enzyme Maps were not different in the bacterial form and the L-forms.These were shown that base alignment of cell wall deficiency on the Cryptic Plasmid B would not change by Restriction Enzyme of HindⅢ,HinfⅠ,MspⅠand HpaⅡ.

关 键 词：细胞壁缺陷型 淋病奈瑟菌 cppB基因 酶切 

分 类 号：R759.2[医药卫生—皮肤病学与性病学]