阴沟肠杆菌的Ⅰ类整合子检测及相关耐药基因分析  

Analysis of drug restance genes in tegrated by class Ⅰ integron in clinical Enterobacter cloacae isolates

作　　者：李岩[1] 苏建荣[1] 许淑珍[1] 刘志远[1] 

机构地区：[1]首都医科大学附属北京友谊医院临床检验中心,北京100050

出　　处：《中国实验诊断学》2010年第9期1356-1358,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的对临床分离的阴沟肠杆菌进行Ⅰ类整合子及相关耐药基因分析,探讨Ⅰ类整合子与阴沟肠杆菌耐药播散的关系。方法使用Vitek-AMS鉴定细菌;采用PCR扩增技术对86株阴沟肠杆菌进行Ⅰ类整合子与ESBLs耐药基因型检测;Ⅰ类整合子与耐药基因水平传播采用质粒接合试验;根据CLSI指南进行药敏试验。结果 86株阴沟肠杆菌中,77.9%的阴沟肠杆菌携带不同大小的整合子,从1 500 bp整合子中检出的耐药基因盒包括:aadB、aadA2;2 500bp整合子中检出PSE-1、aac6-Ⅱ、aadA4基因盒。结论阴沟肠杆菌中常携带含有多种耐药基因的Ⅰ类整合子,Ⅰ类整合子常参与耐药基因在菌株间进行的水平传递。Objective To study distribution of the class Ⅰ intergron in clinical Enterobacter cloacae isolates.Methods E.cloacae was identified by Vitek-AMS;Detection of class Ⅰ intergron and Extended-spectrumβ-lactamases（ESBLs）resistant genes were performed by PCR.Plasmid conjugation test was used to determine the transmission of class Ⅰ intergron.The antibotic susceptibility of Enterobacter cloacae was detected according to the CLSI guideline.Results The incident rate of Enterobacter cloacae with class Ⅰ intergron was 77.9% in 86 strains.Sequencing data of integrons revealed that amplicon 1 500 bp harbored genes of addB and aadA2,amplicon 2 500 bp harbored genes of PSE-1、,aac6-II、,aadA4.Conclusion Emergence of class Ⅰ integrons are frequent in E.cloacae,which usually harbor several different drug-resistant gene casettes.Drug restance genes in tegrated by class Ⅰ integron were widespread in clinical Enterobacter cloacae isolates.

关 键 词：阴沟肠杆菌 Ⅰ类整合子 耐药 

分 类 号：R378[医药卫生—病原生物学]