人血清IgG类抗LDH-C4抗体的ELISA检测及其意义  

ELISA method for detection of IgG-type anti-sperm specific lactate dehydrogenase antibody in human sera and its clinical significance

作　　者：陈平[1] 兰风华[1] 辛娜[1] 张朵[1] 陈勇[1] 苏东辉[2] 

机构地区：[1]南京军区福州总医院分子医学研究中心,福建福州350025 [2]福建医科大学免疫学系,福建福州350004

出　　处：《中国实验诊断学》2010年第9期1399-1401,共3页Chinese Journal of Laboratory Diagnosis

基　　金：福建省科技攻关重点课题(2002Y032)

摘　　要：目的建立检测人血清IgG类抗精子特异性乳酸脱氢酶(LDH-C4)抗体的ELISA法,探讨人血清抗LDH-C4抗体与免疫性不育的关系。方法以重组人LDH-C4(rhLDH-C4)作为包被抗原,建立人血清IgG类抗LDH-C4抗体检测的间接ELISA方法。应用该方法检测74例健康人群(正常生育组)和177例不育人群(不育组)血清IgG类抗LDH-C4抗体。结果初步建立了检测人血清IgG类抗LDH-C4抗体的间接ELISA方法。不育组血清IgG类抗LDH-C4抗体阳性率(30.51%)显著高于正常生育组(9.46%)(P<0.01),女性不育组阳性率(31.46%)与男性不育组(29.55%)差异不显著(P>0.05)。结论人血清中存在着IgG类抗LDH-C4抗体,此类抗体可能与免疫性不育有密切关系。Objective To establish an ELISA method for the detection of IgG-type anti-sperm specific lactatedehydrogenase antibody in human sera and investigate the relationship between anti-sperm specific lactate dehydrogenase antibody and immune infertility.Methods An indirect ELISA was established using recombinant human sperm-specific dehydrogenase（rhLDH-C4） antigen for plate coating and anti-LDH-C4 antibody（IgG type） in the sera from 74 fertile individuals（fertile group） and 177 unexplained infertile patients（infertile group） was detected.Results An indirect ELISA method for detecting IgG type antiLDH-C4 antibody in human sera was established.Of 177 infertile patients（in infertile group）,54 were found positive for serum IgG type anti-LDH-C4 antibody,resulting in a positive ratio of 30.51%（54/177）,which was significantly higher than that in fertile group（7/74 or 9.46%,P0.01）.Within the infertile group,no significant difference of positive ratios were observed between female and male members（31.46% vs 29.55%,P0.05）.Conclusion There was IgG-type anti-LDH-C4 antibody in human sera,and this antibody might be closely related to infertility.

关 键 词：抗精子抗体 乳酸脱氢酶C4 免疫性不育 酶联免疫吸附测定法 

分 类 号：R446.62[医药卫生—诊断学] R711.6[医药卫生—临床医学]