MPP^+对PC12细胞中细胞色素c和caspase-3表达的影响及镇肝熄风汤的调控作用  

Expression of caspase-3 mRNA and Cyt c protein in PC12 cell induced by MPP^+ and the Effect of Cerebrospinal Fluid containing Zhenganxifeng decoction

作　　者：耿玉涛[1,2] 李佳鸣[1,2] 王刚[1,2] 邹德佳[1,2] 李成冲[1,2] 李强[1] 徐浩[1] 牛英才[1] 

机构地区：[1]齐齐哈尔医学院医药科学研究所,黑龙江齐齐哈尔161042 [2]佳木斯大学药学院黑龙江省生物药制剂重点实验室,黑龙江佳木斯154007

出　　处：《辽宁中医杂志》2010年第9期1834-1836,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：黑龙江省自然科学基金资助项目(ZA006-1);黑龙江教育厅科学技术研究面上项目(11541413)

摘　　要：目的:研究甲基-苯基-吡啶离子(1-methyl-4-phenylpyridinium,MPP+)对PC12细胞细胞色素C(Cytc)和caspase-3蛋白表达的影响及镇肝熄风汤载药脑脊液的干预作用。方法:不同浓度的镇肝熄风汤载药脑脊液预处理体外培养的PC12细胞后30min后,加入250μmol/L MPP+共孵育24h。采用实时定量PCR检测PC12细胞caspase-3mRNA的表达,采用ELISA法检测PC12细胞细胞浆中Cyt c浓度变化。结果:MPP+诱导24h,PC12细胞中caspase-3mRNA表达明显增多,细胞浆中Cyt c浓度也明显升高,而镇肝熄风汤载药脑脊液能剂量依赖性地抑制MPP+诱导的caspase-3mRNA表达增多和细胞浆中Cyt c浓度升高。结论:MPP+诱导PC12细胞caspase-3mRNA表达和Cyt c浓度的增加,镇肝熄风汤其增加具有抑制作用。Objective：To investigate the Expression of caspase -3 mRNA and Cyt c protein in PC12 cell induced by 1-methyl-4-phenylpyridinium ion （ MPP ＋ ） and the Effect of Cerebrospinal Fluid containing Zhenganxifeng decoction. Methods：Apoptosis was induced by addition of MPP ＋ into PC12 cell cultures. And different concentration of CSF -ZGXF were administrated 24 h before addition of MPP ＋ into PC12 cell cutrure. caspase-3 mRNA mRNA expression was assayed by real-time PCR. Cyt c protein protein was assayed by ELISA. Results：Expression of caspase-3 mRNA and Cyt c protein in PC12 cell treated with MPP ＋ for 24 h was up-regulated compared with the control group. Expression of caspase-3 mRNA and Cyt c protein in PC12 cell were down-regulated in CSF-ZGXF group compared with the MPP ＋ group on concentration dependent. Conclusion：The results suggest that Zhenganxifeng decoction inhibit expression upregulation of caspase-3 mRNA and Cyt c protein induced by MPP ＋ ,and could be used as an effective drug for Parkinson disease.

关 键 词：镇肝熄风汤 脑脊液药理学 PC12细胞 细胞凋亡 帕金森病 

分 类 号：R285.5[医药卫生—中药学]