细胞色素c蛋白序列分析与结构比较  

Sequence analysis and structure comparison of cytochrome c proteins

作　　者：王中华[1] 王伟[1] 李洋[1] 林英武[2] 黄仲贤[3] 

机构地区：[1]西华师范大学化学化工学院,四川南充637002 [2]南华大学化学化工学院,湖南衡阳421001 [3]复旦大学化学系,上海200433

出　　处：《生物信息学》2010年第3期274-278,共5页Chinese Journal of Bioinformatics

基　　金：西华师范大学科研基金(07B011)资助项目

摘　　要：首先以马心细胞色素c(Horse Cytc)蛋白的氨基酸序列为查询序列,利用生物信息学方法进行相似性搜索,获得了一系列细胞色素c(Cytc)蛋白的氨基酸序列,然后对Cytc蛋白进行了多重对齐分析、进化分析和三维结构比较分析。分析结果表明:Cytc中某些特定部位的氨基酸残基高度保守;相近物种来源的Cytc具有较近的亲缘关系,而来源于同一物种不同部位的Cytc却具有较远的亲缘关系;来源于不同物种的Cytc,即使具有较远的亲缘关系,却具有极其相似的三维空间结构。这些研究结果将为基于Cytc进行蛋白分子设计与构建提供指导意义。A series of cytochrome c（Cyt c） proteins were retrieved by means of bioinformatics methods based on the amino acid sequence of horse heart cytochorme c.Then the obtained protein sequences of Cyt c were analyzed by alignment,evolution and 3D structure comparison.Analytical Results show that some amino acids are highly conservative in cytochromes c（Cyts c）;Cyts c of the same tissues from similar species have related genetic relationship,but Cyts c of different tissues from the same species have remote relationship;Cyts c from various species,even though they have ulterior sibship,have very similar tertiary structures.These results would shed light on the study of protein design construction based on Cyt c.

关 键 词：细胞色素C 蛋白序列分析 分子设计 生物信息学 

分 类 号：Q51[生物学—生物化学]