4-ATP自组膜的重构对辣根过氧化物酶固定化的影响  

Effect of the transformation of 4-aminothiophenol SAMs on immobilization of horseradish peroxidase on gold surfaces

作　　者：冯春梁[1] 杨迎麟[1] 邹文祥[1] 毛晓旭[1] 李丽[1] 

机构地区：[1]辽宁师范大学化学化工学院功能材料化学研究所,辽宁大连116029

出　　处：《辽宁师范大学学报（自然科学版）》2010年第3期344-348,共5页Journal of Liaoning Normal University:Natural Science Edition

基　　金：国家自然科学基金项目(60572009);辽宁省科技攻关项目(2005220024);辽宁省科技基金项目(99102004);辽宁省教育厅高校重点实验室项目(2008S134)

摘　　要：对氨基苯硫酚自组装单分子膜(4-ATPSAMs)具有高度的各向异性,与烷基硫醇相比分子间具有较强的相互作用,以及由于苯环上的离域电子使其具有良好的导电性,因而4-ATPSAMs修饰贵金属表面已引起人们的广泛关注.但是4-ATPSAMs的电化学重构将影响蛋白质在金电极上的固定量.笔者通过制备4-ATP与4-BDT混合硫醇自组膜(MSAMs)以控制4-ATPSAMs的电化学重构,利用电化学技术及傅立叶变换红外光谱研究了4-ATPSAMs的电化学重构对辣根过氧化物酶在金电极上固定化的影响.实验结果表明,MSAMs可有效控制4-ATPSAMs的电化学重构,可为调控界面的物理性质和化学性质提供一种有效手段,对生物传感器和生物芯片的研究与应用具有重要意义.The self-assembled monolayer of 4-aminothiophenol（4-ATP SAMs）have been used in recent years to modify the coinage metal surfaces for a variety of applications.The 4-ATP SAMs are interesting for several reasons.the molecules are highly anisotropic,and the intermolecular interactions are expected to be stronger than those between the alkanethiols,which may lead to different molecular packing structure.Higher electrical conductance would be expected in the aromatic thiol,as the electrons are delocalized in the benzene ring.However,the electrochemical transformation of 4-ATP SAMs will adversely affect immobilization of horseradish peroxidase on gold electrodes.In this paper,the mixed self-assembled monolayer（MSAMs）of 4-aminothiophenol and 4-BDT are used to control the electrochemical transformation of 4-ATP SAMs.The effect of the electrochemical transformation of the SAMs of 4-aminothiophenol on immobilization of horseradish peroxidase of gold surfaces has been studied by means of electrochemical and FTIR measurements.The results indicate that the MSAMs can not only control the electrochemical transformation of 4-ATP SAMs effectively,but also provide the mean to control the chemical and physical properties of the interfaces for a variety of heterogeneous processes,which have a great significance for investigations and applications of biosensors.

关 键 词：自组装单分子膜 对氨基苯硫酚 对巯基苯硫酚 辣根过氧化物酶 生物传感器 

分 类 号：O657.1[理学—分析化学]