不同昆虫泛素基因部分序列的生物信息学分析  

Bioinformatics Analysis of Partial Sequences of Ubiquitin Gene Among Different Insects

作　　者：强承魁[1] 杜予州[2] 凤舞剑[1] 王胜永[1] 周保亚[1] 胡忍[3] 崔亚东[4] 

机构地区：[1]徐州生物工程职业技术学院农林工程系,徐州221006 [2]扬州大学应用昆虫研究所,扬州225009 [3]徐州生物工程职业技术学院科技产业处,徐州221006 [4]阜阳师范学院生命科学学院,阜阳236032

出　　处：《生物技术通报》2010年第10期188-192,197,共6页Biotechnology Bulletin

基　　金：徐州生物工程高等职业学校校长基金项目(200901);安徽省农业科学院院长青年创新基金项目(2009A0908);国家重点基础研究发展规划("973"计划)项目(2006CB102002)

摘　　要：采用比较基因组学和生物信息学方法系统分析了NCBI中已公布的二化螟、棉卷叶螟和草地贪夜蛾等13种昆虫Ub基因及其氨基酸序列的结构特点、差异和遗传多样性及进化关系。结果表明,Ieu、Thr、Ile和Lys作为13种昆虫Ub的主要氨基酸,多呈碱性,无前导肽、信号肽和跨膜结构域,延伸带和无规则卷曲为主要结构元件,第57和22位点分别发生Ser和Thr磷酸化,总体呈亲水性。同时检测出96个多态位点,共生成13个单倍型,昆虫种间该基因具较丰富的遗传多样性。Based on the published sequences of ubiquitin gene of Chilo suppressalis, Haritalodes derogata and Spodoptera frugiperda and so forth in NCBI,the structure characters,differences and genetic diversity were analyzed using the method of comparative genomics and bioinformatics. The resuhs showed that the main amino acids were Ieu,Thr,Ile and Lys,and most of basic amino acids. Prepropeptide,signal peptide and transmembrane domain were not found. The main structural element was extended strand and random coil in the predicted secondary structure. Phosphorylation sites of Ser and Thr occurred in the amino acid residue 57 and 22, respectively. The total of 96 polymorphic sites were detected from 13 sequences and 13 hapolotypes were sorted. More nucleotide diversity and amino acid diversity of ubiquitin gene existed among 13 insects.

关 键 词：昆虫 泛素基因 生物信息学 遗传变异 

分 类 号：Q96[生物学—昆虫学]