基于玉米BC_2F_2群体的穗部性状QTL分析  

QTL Analysis of Ear Traits Based on BC_2F_2 Population in Maize (Zea may L.)

作　　者：胡利宗[1] 刘均革 郭晋杰[1] 赵永锋[1] 祝丽英[1] 宋占权[3] 陈景堂[1] 

机构地区：[1]河北农业大学,河北保定071001 [2]天津静海良种场,天津301600 [3]保定学院,河北保定071051

出　　处：《华北农学报》2010年第4期73-77,共5页Acta Agriculturae Boreali-Sinica

基　　金：河北省"十一五"科技支撑计划(20061148001);河北省自然科学基金项目资助(C2006000441);河北省教育厅自然科学研究项目(Z2008115)

摘　　要：以农系110为受体、农系531为供体材料,采用回交法构建了样本容量为95株的BC2F2回交群体,选取126个均匀分布在10条染色体上的多态性SSR标记进行6个穗部性状QTL分析。结果表明,受体农系110的穗长、穗粗、穗行数、行粒数、百粒重和穗粒重6个穗部性状表型值分别增加了9.4%,9.6%,4.1%,2.7%,0.35%和4.4%;构建一张含126个SSR标记的玉米分子标记连锁遗传图谱,总长度为2317.4cM,平均区间长18.4cM;采用复合区间作图法,定位了19个与玉米穗部性状有关的QTL,其中穗长QTL4个,穗粗QTL2个,行粒数QTL1个,穗行数QTL3个,百粒重QTL4个,穗粒重QTL5个。The mapping population derived from a cross of Nongxi 110 as receptor and Nongxi 531 as donor,including 95 BC2F2 individuals,was developed by back cross breeding program in maize （Zea may L.） . The 126 polymorphic SSR primer pairs between parents,uniformly covering the whole genome,were used for genotyping of 95 BC2F2 individuals. The correlation and OTL analysis for ear traits were performed in this mapping population. The correlative result indicated that there is a significant positive correlation between ear length,ear row number of grain,row kernel number and ear weight,a significant positive correlation between ear length and row kernel number,but a significant negative correlation between ear length and ear diameter. According to CIM mapping method,the total 19 QTL for 6 ear traits were identified and located on in the chromosomes which included 4 QTL for ear length,2 QTL for ear diameter,1 QTL for row kernel number,3 QTL for ear row number,4 QTL for hundred kernel weight,5 QTL for ear kernel weight.

关 键 词：玉米 数量性状位点 穗部性状 作图群体 

分 类 号：S513.03[农业科学—作物学]