北京地方品种鸡禽白血病病毒的跟踪检测与PCR检测方法的建立  

Survey of ALV Status in Beijing Suburb Chicken Population and Development of PCR for Detected ALV

作　　者：王改利[1,2] 刘华贵[1] 初芹[1] 张剑[1] 赵权[2] 张莉[1] 

机构地区：[1]北京市农林科学院畜牧兽医研究所,北京100097 [2]吉林农业大学动物科技学院,吉林长春130118

出　　处：《华北农学报》2010年第4期213-217,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家科技支撑计划项目(2008BADB2B07);国家自然科学基金青年项目(30700595);国家自然科学基金项目(30972048)

摘　　要：采用ELISA方法对京郊某种鸡场2群鸡从出雏到45周龄进行禽白血病病毒p27(ALV-p27)抗原检测,对血清进行ALV-AB及ALV-J抗体检测,按标准计算方法对检测数据进行分析。结果表明:A群鸡不同周龄直肠拭子中ALV-p27抗原检出率差异显著,出雏时为0.73%(2/273),8周龄最高,阳性率达45.06%(114/253);B群鸡不同周龄直肠拭子中ALV-p27抗原检出率差异显著,出雏时为0(0/182),14周龄时最高,达45.52%(66/145)。A群鸡38,45周龄ALV-J抗体阳性率分别为35.71%(15/42),27.08%(13/48),B群鸡27,34,43周龄ALV-J抗体阳性率分别为4.00%(2/50),2.33%(1/43),9.30%(4/43)。A群鸡45周龄ALV-AB抗体阳性率为8.33%(4/48),B群鸡34,43周龄ALV-AB抗体阳性率分别为6.98%(3/43),6.98%(3/43)。同时建立PCR检测ALV的方法,分别扩增出大小为717bpP27抗原片段和545bp的ALV-J特异性片段,P27片段与ALV-J亚群毒株HPRS-103、NX0101的相应核苷酸序列同源性达91.3%和94.7%,ALV-J特异性片段与ALV-J亚群毒株HPRS-103、NX0101的相应核苷酸序列同源性达97.1%～97.3%和98.1%～98.6%。ELISA was used to detect the P27 antigen of avian leukosis virus （ALV-P27） in fecal swabs of two flocks in one chicken breeder farm in Beijing suburb,and serum samples of corresponding flock were collected for detection antibody of ALV-J and ALV-AB. The results showed that the detection rate of ALV-P27 was significant difference in flock A. The detection rate of ALV-P27 was respectively 0. 73% （2 /273） at 0 day,45. 06% （114/253） at 8 weeks,and the rate of ALV-P27 was 0（0/182） at 0 day and 45. 52% （66/145） at 14 weeks in flock B. The detection rate of antibody of ALV-J was 35. 71% （15/42） ,27. 08% （13/48） in flock A and 4. 00% （2/50） ,2. 33% （1/43） , 9. 30% （4/43） in flock B. The detection rate of antibody of ALV-AB was 8. 33% （4/48） in flock A and 6. 98% （3/43） 、6. 98% （3/43） in flock B. Simultaneously,amplified by PCR and sequenced. The specific fragment of 717 bp for P27 and 545bp for ALV-J was amplified from samples. The amplified 717 bp fragment shared 91. 3% and 94. 7% identities in nucleotide with that of HPRS-103 and NX0101 strains of ALV-J. And 545 bp fragment shared 97. 1% - 97. 3% and 98. 1% -98. 6% identities in nucleotide with HPRS-103 and NX0101 strains of ALV-J.

关 键 词：禽白血病病毒 特异性P27抗原 聚合酶链式反应 

分 类 号：S855.3[农业科学—临床兽医学]