槲皮素键合纳米硅胶对牛血清蛋白吸附的研究  

The absorption behaviour study of quercetin-bonded nano silica gel for bovine serum albumin

作　　者：易翔[1,2] 张剑[1,2] 翟云云[2] 曾延波[2] 李蕾[2] 

机构地区：[1]江苏工业学院化学化工学院,江苏常州213016 [2]嘉兴学院生物与化学工程学院,浙江嘉兴314001

出　　处：《化学研究与应用》2010年第9期1187-1190,共4页Chemical Research and Application

基　　金：浙江省科技计划项目(2007C23071)资助;浙江省自然科学基金项目(Y4080252)资助

摘　　要：A simple method was described for the preparation of quercetin-bonded silica gel using γ-aminopropyltriethoxy silane as coupling reagent.The structure of the ligand was characterizied by Fourier transform infrared spectroscopy,thermal analysis and SEM etc.The stability of QBSG was studied.Some important parameters,such as pH,absorption time,the concentration of Bovine Serum Albumin(BSA) which can influence the absorption of BSA were investigated.The comparative study of absorption for SG and QBSG was also carried.The result shows that the QBSG is stable under strong acid condition.It was also found:when the adsorption pH value was 4,the concentration of the BSA was 0.267 g/L,and the adsorption time was 40 minutes,the QBSG owned the greatest capacity of 22.9 mg/g.The QBSG exhibited higher uptake capacity than SG in the same condition.A simple method was described for the preparation of quercetin-bonded silica gel using γ-aminopropyltriethoxy silane as coupling reagent.The structure of the ligand was characterizied by Fourier transform infrared spectroscopy,thermal analysis and SEM etc.The stability of QBSG was studied.Some important parameters,such as pH,absorption time,the concentration of Bovine Serum Albumin（BSA） which can influence the absorption of BSA were investigated.The comparative study of absorption for SG and QBSG was also carried.The result shows that the QBSG is stable under strong acid condition.It was also found：when the adsorption pH value was 4,the concentration of the BSA was 0.267 g/L,and the adsorption time was 40 minutes,the QBSG owned the greatest capacity of 22.9 mg/g.The QBSG exhibited higher uptake capacity than SG in the same condition.

关 键 词：槲皮素 纳米硅胶 牛血清蛋白 吸附 

分 类 号：O658[理学—分析化学]