白花丹参药材HPLC指纹图谱研究  

HPLC fingerprint of Salvia Miltiorrhiza f. Alba

作　　者：王福刚[1,2] 高允生[1] 齐永秀[1] 刘斌[2] 王伟[2] 

机构地区：[1]泰山医学院药学院,山东泰安271016 [2]北京中医药大学中药学院,北京100102

出　　处：《中成药》2010年第9期1464-1467,共4页Chinese Traditional Patent Medicine

基　　金：山东省教育厅科技项目(J04E07);泰安市科技局课题(KY200530)

摘　　要：目的:建立白花丹参药材的HPLC指纹图谱。方法:采用高效液相色谱法,色谱柱:Diamonsil C18(4.6mm×250mm,5μm),流动相:甲醇-0.1%三氟乙酸水溶液进行梯度洗脱,检测波长270nm,流速1.0mL/min。结果:在选定的色谱条件下,通过中药色谱指纹图谱相似度评价系统(2004A)确定14个色谱峰构成白花丹参药材指纹图谱的特征峰。结论:该分析方法的稳定性、精密度、重现性较好。】 AIM： To develop an HPLC fingerprint method for the quality control of Salvia Miltiorrhiza f. Alba. METHODS： An HPLC analysis was carried out on a Diamonsil C18 column（4. 6 mm × 250 mm,5 μm） with themobile phase consisting Methanol-0. 1% trifluoroacetic acid water in gradient mode. The detection wavelength was set at 270 nm. The flow rate was 1. 0 mL·min-1 and the analytic time was 80 min. The ten batches of samples were determined,for establishing the standard HPLC fingerprint of Salvia miltiorrhiza f. Alba. RESULTS： Under the above chromatographic conditions the characteristic peak of fingerprint of Salvia miltiorrhiza f. Alba was composed of 14 chromatographic peaks through similarity analysis. CONCLUSION： The method has good reproduc-ibility,precision and stability.

关 键 词：白花丹参 高效液相色谱法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]