半枝莲水提工艺中野黄芩苷降解动力学研究  

Degradation kinetics of scutellarin in water extraction of Herba Seutellariae Barbata

作　　者：李一圣[1] 陈剑平[1] 何耀慧[1] 林荣锋[1] 黄晓其[1] 曾惠芳[2] 苏子仁[1] 

机构地区：[1]广州中医药大学,广东广州510006 [2]广州中医药大学一附院药剂科,广东广州510405

出　　处：《中成药》2010年第9期1498-1500,共3页Chinese Traditional Patent Medicine

摘　　要：目的:研究不同浸泡条件下,半枝莲水提工艺中野黄芩苷含量的变化。方法:采用高效液相法,测定不同温度和浸泡时间下半枝莲水提液中野黄芩苷的含量;以野黄芩苷含量和时间作为变量进行进行动力学拟合并计算参数。结果:在20～60℃浸泡条件下,半枝莲水提液中野黄芩苷含量的变化呈降解特征,以30～40℃降解速度最快;而在70℃以上浸泡,半枝莲水提液中野黄芩苷含量保持不变,提取率较高。结论:半枝莲药材在浸泡的过程中,野黄芩苷存在酶降解反应;水提取时宜在70℃以上投料,可有效地提高野黄芩苷的提取率。AIM： To study the changes of scutellarin in Herba Seutellariae Barbara under different immersion conditions. METHODS： HPLC was adopted to detect the content of scutellarin in the water extraction of Herba Seutellariae Barbata immersed in different temperature and time;the scutellarin content and soaking time were selected as variats of dynamic equation and the kinetics parameters were calculated. RESULTS： Under the immersing conditions of from 20 ℃ to 60 ℃, the changes of scutellarin content significantly tended to degrad in the water extraction of Herba Seutellariae Barbata, and degradation rate was the fastest from 30 ℃ and 40 ℃ ; While the scutellarin content in water extraction remained unchanged and it had a higher extraction rate at the immersion temperature above 70℃. CONCLUSION： Scutellarin of Herba Seutellariae Barbata existes enzymatic degradation reaction in the process of immersion; it can effectively increase the extraction rate of scutellarin at the temperature above 70 ℃.

关 键 词：半枝莲 野黄芩苷 降解 浸泡 

分 类 号：R284.2[医药卫生—中药学]