非O1、非O139型霍乱弧菌中首次发现PER-1超广谱β内酰胺酶  

First identification and genetic analysis of extended-spectrum β-lactamase PER-1 in a strain of non-O1,non-O139 Vibrio cholerae

作　　者：孙景勇[1] 倪语星[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院临床微生物科,200025

出　　处：《中国感染与化疗杂志》2010年第5期338-341,共4页Chinese Journal of Infection and Chemotherapy

摘　　要：目的明确非O1、非O139型霍乱弧菌中ESBL的基因型及其遗传背景,以探讨该耐药基因传播的机制。方法通过表型确证试验确定细菌是否产生ESBL,通过接合试验了解ESBL是否由质粒介导,通过PCR扩增和序列分析确定ESBL的基因型,通过反向PCR和序列分析了解ESBL基因两侧的结构。结果 ESBL表型确证试验确认菌株RJ354为产ESBL菌株,接合试验证实ESBL基因位于可接合质粒上,PCR、反向PCR扩增和序列分析确定其基因型为blaPER-1,该基因上游序列为ORF513,下游为gst-like基因。结论本研究国际上首次在从非O1、非O139型霍乱弧菌中检出了PER-1型ESBL,而且首次发现该酶基因与一种新的基因元件ISCR1相连,后者可能介导前者的水平转移。Objective To identify the genotype and its genetic environment of the extended-spectrum β-lactamase in a non-O1, non-O139 Vibrio cholerae isolate. Methods MICs and ESBLs were determined by E-test following CLSI guidelines. Conjugation experiments were carried out with Escheriehia coli J53Az^r to explore if the ESBL gene was located in plasmid. PCR amplifications were performed using the primers specific for blaeER-1 , blas,v and blavEB, The complete blapER-1 structural gene and its flank regions were determined by inverse PCR. Extended sequence analysis was performed to gain insight into the structure and organization of the blaPER-1 gene area. Results Non-O1, non-O139 Vibrio cholerae RJ354 was an ESBL-producing strain. This ESBL was plasmid mediated, which was confirmed by conjugation experiment. The genotype of the ESBL was blaPER-1. The flank located upstream of the blaPER-1 gene was ISCR1 （ORF513）. The flank located downstream of the blaPER-1 gene was gstlike gene. Conclusions This is the first report that a PER-1 ESBL was detected in a non-O1, non-O139 Vibrio cholerae isolate in the world. It is first found that the blaPER-1 was associated with an ISCR1 element, which may account for horizontal transmission of blaPER in our country.

关 键 词：非O1 非O139型霍乱弧菌 超广谱Β内酰胺酶 PER-1 ISCR1 

分 类 号：R378[医药卫生—病原生物学]