载荷bFGF-PLGA微球的胶原海绵的制备及其体外缓释性能研究  

Preparation of Collagen Sponge Integrated with bFGF-Loaded PLGA Microspheres and Its Sustained Release Performance in Vitro

作　　者：武继民[1,2] 汪鹏飞[2] 李志宏[2] 刘永清[2] 袁晓燕[1] 

机构地区：[1]天津大学材料科学与工程学院,天津300072 [2]军事医学科学院卫生装备研究所,天津300161

出　　处：《天津大学学报》2010年第7期629-632,共4页Journal of Tianjin University(Science and Technology)

基　　金：国家自然科学基金资助项目(30970724);天津市自然科学基金资助项目(08JCYBJC03400)

摘　　要：在支架材料上引入具有控释行为的生长因子旨在结构与功能上模拟细胞外基质能.制备了一种载荷bFGF-PLGA微球的胶原海绵缓释体系,探讨其在体外对bFGF的释放性能.采用复乳溶剂挥发法制作微球,将微球与胶原海绵复合;通过扫描电镜观察微球和载荷微球胶原海绵的表面形态结构;利用ELISA法测试微球中药物的载药量和包封率,并对微球和载荷微球胶原海绵中bFGF的体外释放行为进行研究.结果表明:微球表面圆滑,载药量为0.059 9%±0.001 9%,包封率为79.9%±2.8%;载荷微球海绵的突释率为14.6%,低于微球本身20.0%的水平;而且载荷微球海绵的体外释放bFGF时间比微球本身更长.载荷bFGF-PLGA微球的胶原海绵缓释体系在体外能够稳定地缓释bFGF,具有较小的突释率,有望发展成为一种理想的组织工程支架材料.Growth factors with sustained release have been introduced into scaffolds to simulate the structure and function of extracellular matrix. Collagen sponge integrated with bFGF-loaded PLGA microspheres has been prepared and its sustained-release performance for bFGF in vitro is studied. The bFGF-loaded PLGA microspheres were pre- pared with multiple emulsion volatilizing method and integrated with collagen sponge. The morphology of the micro- spheres with and without collagen sponge was investigated using scanning electron microscope （SEM）. ELISA method was used to detect the drug loading amount and encapsulation efficiency of the microspheres and to study the sustained-release behavior of the microspheres with and without collagen sponge for bFGF in vitro. The results show that the smooth microspheres have drug loading amount and encapsulation efficiency of 0.059 9%~ 0.001 9% and 79.9% ~： 2.8%, respectively. The complexes can steadily release bFGF for a longer time and have bFGF initial burst release of 14.6%, less than the microspheres of 20.0%. With the steady release of bFGF in vitro and relatively low initial burst release rate, collagen sponge integrated with bFGF-loaded PLGA microspheres has great potential to be a satisfactory material for tissue engineering.

关 键 词：碱性成纤维细胞生长因子 PLGA微球 缓释 胶原海绵 

分 类 号：R318[医药卫生—生物医学工程]