番木瓜叶片总RNA提取方法比较研究  

Comparative Study of RNA Extraction From on The Papaya

作　　者：陈萍[1] 姜成东[1] 卢业凌[1] 

机构地区：[1]海南大学热带作物种质资源保护与开发利用教育部重点实验室,海南大学园艺园林学院,海南儋州571737

出　　处：《北方园艺》2010年第18期133-135,共3页Northern Horticulture

基　　金：海南大学校基金资助项目(hd09xm62);海南省教育厅高等学校科学研究资助项目(Hjkj2010-17);(原)华南热带农业大学科技基金资助项目(Rnd0710)

摘　　要：采用了CTAB法和GHCL法及SDS法3种方法分别对番木瓜的叶片进行RNA的提取。该试验采用SDS法、GHCL法提取番木瓜总RNA,并对提取的RNA进行质量比较。结果表明:GHCL法(方法2)提取的RNA产率高,无明显降解,OD260/OD280比值较好,是有效提取总RNA的方法。Total RNA of Caricap apayawas extracted by means of three different methods.They were CTAB method and GHCL method and SDS method.The quality of extraction RNA by these methods had been compared.The results showed that,the GHCL method（method two）was an effective way,degradation was little.This was the better method since the RNA extracted the OD260/OD280ratio was better.

关 键 词：番木瓜 RNA 提取方法 

分 类 号：S662.2[农业科学—果树学]