根癌农杆菌介导外源基因转化番茄体系优化  

The Condition Optimization for Tomato Gene Transform by Agrobacterium Tumefaciens-introduced

作　　者：潘永明[1] 

机构地区：[1]牡丹江师范学院,黑龙江牡丹江157012

出　　处：《北方园艺》2010年第18期142-144,共3页Northern Horticulture

基　　金：牡丹江市科技局科研资助项目(G2009n2015)

摘　　要：为建立优化的番茄外源基因转化体系,提高番茄的外源基因转化率,以番茄叶盘预培养时间和农杆菌与番茄叶盘的共培养温度、共培养时间为试验要素,摸索相对适宜的预培养及共培养的时间和温度范围,其余操作按常规步骤进行。结果表明:农杆菌与番茄叶盘的预培养适宜时间为27℃下32～48h;农杆菌与番茄叶盘的共培养的最适宜条件是:22～24℃下暗培养48h。It was used as the experimental essential factor that the pre-culture time of tomato leaves and the co-culture time and temperature of the agrobacterium tumefacien and the tomato leaves of grass plate altogether raise,then designed experiment.Tried to find out relative suitable time and temperature range,other operations carry on according to the conventional operation,the empirical datum indicated that the agrobacterium tumefacien and the tomato leaves of grass plate pre-culture time was 32-48hours;the most suitable condition of agrobacterium tumefacien and the tomato leaves of grass plate was：cultured in 22-24℃under raises darkly for 48hours.

关 键 词：番茄 根癌农杆菌 体系优化 

分 类 号：S641.2[农业科学—蔬菜学]