观赏贝母种质亲缘关系的ISSR分析  

Analysis of Genetic Relationship Ornamental Fritillaria Germplasm Resources by ISSR Markers

作　　者：吴晓清[1,2] 成海钟[1] 周玉珍[1] 娄晓鸣[1] 张文婧[1] 汤庚国[2] 

机构地区：[1]苏州农业职业技术学院,江苏苏州215008 [2]南京林业大学,江苏南京210037

出　　处：《北方园艺》2010年第18期157-160,共4页Northern Horticulture

基　　金：江苏省农业三项工程资助项目(SX(2007)907);苏州市自然科学基金资助项目(YJG0906)

摘　　要：利用ISSR标记对22份观赏贝母材料进行了基因组多态性分析,从100条引物中筛选出13条用于观赏贝母的ISSR扩增。共扩增出178条带,其中多态性条带160条,多态性百分率为90%。根据ISSR扩增结果,利用NTSYSpc2.10e软件进行Jaccard相似性系数分析,22份观赏贝母的遗传相似系数为0.2338～0.8788。通过UPGMA进行聚类分析,探索亲缘关系。在遗传相似系数0.50处,22份观赏贝母材料可分为二大类群。第一大类包括比较原始的贝母组和特化的聚花组和单鳞组的贝母;第二大类包括演化水平较高的多鳞组的3种贝母。由此可见,同一组观赏贝母种质之间表现较近缘的亲缘关系。22 Ornamental Fritillariagermplasm were used as materials for analyzing their genome polymorphism by ISSR markers.13primers selected from 100primers were used for ISSR amplification.A total of 178bands were generated,of which 160bands were polymorphic bands（the percentage of polymorphic band,PPB=90%）.According to the results of ISSR amplification,which were analyzed to Jaccard similarity coefficient by NTSYSpc 2.10esoftware,the genetic similarity coefficient was 0.2338to 0.8788.The clustering dendrogram was constructed by UPGMA method.22Ornamental Fritillaria materials were divided into two major groups while the similarity coefficient was 0.50.The first group included original Sect.Fritillaria,evolvedS ect.Petilliumand Sect.Theresia.The second group included 3ornamental Fritillariain more evolved Sect.Liliorhiza.The species in one sect had closer phylogenetic relationship.

关 键 词：观赏贝母 ISSR 亲缘关系 聚类分析 

分 类 号：S682.29[农业科学—观赏园艺]