类圆环病毒因子P1感染对猪外周血单个核细胞抗病毒蛋白mRNA转录的影响  

Effects of Porcine Circovirus-like Agent P1 Infection on the Porcine Antiviral Protein mRNA Transcriptions in Peripheral Blood Mononuclear Cells of Piglets

作　　者：温立斌[1] 何孔旺[1] 杨汉春[2] 郭容利[1] 俞正玉[1] 茅爱华[1] 倪艳秀[1] 张雪寒[1] 周俊明[1] 吕立新[1] 李彬[1] 王小敏[1] 

机构地区：[1]江苏省农业科学院兽医研究所,农业部动物疫病诊断与免疫重点开放实验室,国家兽用生物制品工程技术研究中心,江苏南京210014 [2]中国农业大学动物医学院,农业部预防兽医学重点开放实验室,北京100094

出　　处：《华北农学报》2010年第B08期7-11,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金(30972184);江苏省自然科学基金(BK2008351)

摘　　要：为探讨类猪圆环病毒2型因子P1感染对猪抗病毒蛋白分子mRNA转录的影响,将P1分子克隆接种30日龄健康广西巴马小型猪,利用实时荧光定量PCR技术对猪外周血单个核细胞抗病毒蛋白分子PKR、2'-5'OAS2、 RNase L和Mx1的mRNA转录水平的变化进行了定量分析。结果表明,PKR mRNA转录在接种后8 d和40 d呈上调趋势;而2'-5'OAS2 mRNA在3 d和17 d下调,Mx1 mRNA也在接种后17 d下调;RNaseL mRNA转录水平则没有发生明显变化。研究揭示P1感染猪后,机体的抗病毒蛋白的功能受到了不同程度的影响。The study was to explore the effects of porcine circovirus-like agent P1 infection on the porcine anti-viral protein mRNA transcriptions.About thirty-day-old healthy Guangxi Bama miniature pigs were inoculated with cloned genomic DNA of P1.The changes of mRNAs of porcine antiviral protein（PKR,2＇-5＇OAS2,RNase L and Mx1） in peripheral blood mononuclear cells（PBMC） of piglets were analyzed by real-time fluorescent quantitative PCR（real-time FQ-PCR） .The results showed that the transcriptions of PKR mRNAs were increased in the affected piglets at 8 and 40 day post-inoculation（DPI） ,respectively.Whereas 2＇-5＇OAS2 mRNA levels of the inoculated pigs were downregulated at 3 DPI and 17DPI,the P1 infected group trended to decrease expression of Mx1 mRNA at 17DPI as well.RNase L mRNA expressions appeared to be no significant change by P1 infection.In conclusion,the results indicated that P1 infection can affect the mRNA levels of PKR,2＇-5＇OAS2 and Mx1.

关 键 词：P1 外周血单个核细胞 抗病毒蛋白 

分 类 号：Q78[生物学—分子生物学]