甜瓜育种亲本材料遗传多样性及群体结构的SSR标记分析  

Analysis of Genentic Diversity and Population Structure in Melon (Cucumis melo L.) Breeding Materials by SSR Markers

作　　者：王美荣[1,2] 许勇[2] 詹永乐[1] 郭绍贵[2] 任毅[2] 宫国义[2] 张海英[2] 

机构地区：[1]安徽农业大学生命科学院,安徽合肥230036 [2]北京市农林科学院蔬菜研究中心,北京100097

出　　处：《华北农学报》2010年第B08期41-46,共6页Acta Agriculturae Boreali-Sinica

基　　金：北京市科委项目(Z09090501040902;D08070500690803);国家现代西瓜甜瓜产业技术体系(nycytx-36-01-01-01);国家科技支撑项目(2006BAD01A7-6-02)

摘　　要：为明确供试甜瓜育种材料间的遗传关系,为育种工作提供有益信息,利用23对SSR引物对39份甜瓜育种材料(双亲、F1)进行遗传多样性和群体结构分析,共检测到71等位变异位点,平均每对引物产生等位变异数3.087个、Shannon-Weaver指数为0.893。遗传多样性和群体结构分析结果基本一致,供试育种材料分为厚皮甜瓜和薄皮甜瓜两大类群。群体结构分析还阐明了各育种材料的遗传组成,84.62%的育种材料血缘相对比较单一,15.38%的育种材料拥有混合来源。研究结果为亲本选配、优良育种材料利用提供了技术支持。To explore the genetic background of melon breeding materials,23 polymorphic Simple sequence repeat（SSR） markers were conducted to assess the genetic diversity and population structure among 39 melon breeding materials.71 allelic variations were detected,the average allele number and Shannon-Weaver index of each primer were 3.087 and 0.893.Genetic diversity and population structure analyses revealed that results were basically same,39 melon accessions were clustered into two groups,Cucumis melo ssp.melo and C.melo ssp.conomon.The population structure analyses revealed the genetic composition of the 39 accessions,in which 84.62%accessions shared a likely single ancestor,and the rest were from mixed ancestors.These results had provided valuable information for breeding and exploit of new melon accessions.

关 键 词：甜瓜 育种材料 SSR 遗传多样性 群体遗传结构 

分 类 号：S652.03[农业科学—果树学]