一种新型玉米苗期反复干旱存活率鉴定试验设计及效果研究  

A New Design Method of Double Randomized Block with Single Planting for Evaluating Seedling Survival Percentage after a Repeated Drought Stress in Maize

作　　者：姚艳荣[1] 贾秀领[1] 马瑞昆[1] 贾银锁[2] 张丽华[1] 董志强[1] 申海平[1] 郭丽[1] 

机构地区：[1]河北省农林科学院粮油作物研究所,河北石家庄050031 [2]河北省农林科学院遗传生理所,河北石家庄050051

出　　处：《华北农学报》2010年第B08期148-151,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家科技支撑计划(2007BAD69B01;2007BAD69B04)

摘　　要：为改进玉米苗期反复干旱存活率法鉴定苗期抗旱性的试验设计。采用双重随机区组单株设计法鉴定了32个玉米品种的苗期抗旱性,结果表明,本设计使不同鉴定品种在干旱-复水过程中所处的局部微环境内土壤水分含量均匀一致,提高了鉴定结果准确性和可操作性,鉴定结果重复性好。同时,一次干旱-复水与两次干旱-复水的幼苗存活率极显著正相关。提出了采用苗期反复干旱存活率鉴定玉米苗期抗旱性的一种新型设计-双重随机区组单株设计,建立了相应的技术规程。提出采用一次干旱复水存活率鉴定玉米苗期抗旱性更加实用高效。Seedling survival percentage after a repeated drought stress has been extensively used as an evaluation index for seedling drought resistance in many crops.This paper aimed at improving the method for identifying seedling survival percentage after a repeated drought stress for seedling drought resistance evaluation in maize crop. A new type experiment design,termed here as double randomized block with single planting,was proposed and used to identify seedling survival percentage after a repeated drought stress in 32 varieties in maize.The experiment was conducted in separated plots compartted by concrete walls on sides and bottom.Maize seedling was germinated in sand medium and transplanted into the plot to ensure uniformly vigorous seedlings.It has been proved that the experiment design has assured uniform soil moisture for seedlings of all varieties,and that sand cultures technique effectively improved the seedling vigor uniformity.Both factors have contributed to the satisfactory identification results with acceptable variations among replications.The experiment revealed a significant linear positive correlation（r= 0.913,n=32） between seedling survival percentage after the first drought-rehydrtion circle and that after the second drought-rehydration circle.A new type experiment design-double randomized block with single planting-in maize was proposed,with a draft of rules for drought resistance identification technique,which greatly reduced the variations in soil moisture among different varieties resulted from difference in plant leaf area of seedlings.It has also been suggested that seedling survival percentage after the first drought-rehydration cycle can be used as an effective and practical method for identification and evaluation of maize seedlings.

关 键 词：玉米 苗期抗旱性 反复干旱存活率 双重随机区组单株设计 

分 类 号：S513.01[农业科学—作物学]