牡蛎壳纳米羟基磷灰石的制备与表征  

Preparation and Characterization of Nano-hydroxyapatite from Oyster Shell

作　　者：蔡汝汝[1] 杨云[1] 孙珍珍[1] 姚清清[1] 侯振清[1] 张其清[1,2,3] 

机构地区：[1]厦门大学材料学院,福建省生物医学工程重点实验室,福建厦门361005 [2]中国医学科学院,北京协和医学院,北京100730 [3]天津市生物医学材料重点实验室,天津300192

出　　处：《厦门大学学报（自然科学版）》2010年第5期666-670,共5页Journal of Xiamen University：Natural Science

基　　金：“十一五”国家科技支撑计划重点项目(2007BAD07B05);福建省海洋与渔业局重点项目(闽海渔合同号[2008]1-11)

摘　　要：以僧帽牡蛎壳粉末为原料,通过水热反应合成纳米羟基磷灰石(n-HA),考察反应时间、反应温度、反应物摩尔比及牡蛎壳粉末的微结构对反应的影响.运用X射线衍射(XRD)、扫描电子显微镜(SEM)、红外光谱(FTIR)、X射线能谱仪(EDS)及MTT实验表征产物的晶相组成、形貌、化学组成及细胞相容性.结果显示,牡蛎壳粉末经水热反应后仍保持其原有形貌,产物为部分CO32-取代的片状n-HA,其Ca/P摩尔比约为1.5,其结构及组成与人骨HA相似,细胞相容性良好.牡蛎壳粉末外表面方解石通过溶解重结晶过程转化为片状n-HA,内部方解石则经由固态局部规整离子交换反应转化为n-HA.最佳水热反应条件为220℃下反应6 h,牡蛎壳中Ca与(NH4)2HPO4中P摩尔比为5∶6.Nano-hydroxyapatite（n-HA） from Ostrea cucullata Born was obtained by hydrothermal synthesis method.The influence of reaction time,temperature,molar ratio of reactants and microstructure of oyster shell powder on the reaction was investigated.X-ray diffraction（XRD）,scanning electron microscopy（SEM）,Fourier transform infrared spectroscopy（FTIR）,energy dispersive spectroscopy（EDS） and MTT were employed to characterize the products.The results indicated that sheet structure of carbonated n-HA were successfully produced via hydrothermal transformation.n-HA retained the morphology of the original calcite.The molar ratio of Ca/P was about 1.5.The structure and component were much more similar to bone HA with good cell compatibility.The exterior calcite converts to n-HA via dissolution-recrystallization.The interior calcite converts to n-HA by a solid-state topotactic ion-exchange reaction.The best hydrothermal transformation condition is reacting at 200 ℃ for 6 h,the molar ratio of Ca in oyster shell to P in（NH4）2HPO4 is 5∶6.

关 键 词：牡蛎壳 纳米羟基磷灰石 水热反应 反应机理 生物相容性 

分 类 号：R318[医药卫生—生物医学工程]