壳聚糖/羟基磷灰石复合骨修复材料的制备及细胞相容性初步研究  

Preparation and Cell Compatibility of Chitosan-hydroxyapatite Composite for Bone Repair

作　　者：孙珍珍[1] 蔡汝汝[1] 蒲曦鸣[1] 杨云[1] 姚清清[1] 张其清[1,2,3] 

机构地区：[1]厦门大学材料学院,福建省生物医学工程重点实验室,福建厦门361005 [2]中国医学科学院,北京协和医学院,北京100730 [3]天津市生物医学材料重点实验室,天津300192

出　　处：《厦门大学学报（自然科学版）》2010年第5期671-675,共5页Journal of Xiamen University：Natural Science

基　　金：“十一五”国家科技支撑计划项目(2007BAD07B05);福建省海洋与渔业局重点项目(闽海渔合同号[2008]1-11)

摘　　要：采用原位沉析法以壳聚糖(CS)为基体,乙酸钙及磷酸作为钙磷源制备壳聚糖/羟基磷灰石(CS/HA)复合棒材,并用三聚磷酸钠(TPP)-磷酸钠(TSP)混合溶液进行交联.通过燃烧试验、FTIR、XRD、TEM和弯曲强度测试对材料进行理化表征,MTT法检测材料的细胞相容性.燃烧试验结果表明,无机成分均匀分散于CS中,FTIR、XRD和TEM测试结果证实原位生成HA.经弯曲强度测试,复合材料弯曲强度达133 MPa,弯曲模量6.8 GPa.MTT实验结果表明,材料可以促进成骨细胞增殖,具有良好的细胞相容性.所制得的CS/HA复合棒材力学性能得到很大提高,并且具有良好的细胞相容性,有望作为一种新型的骨折内固定材料.Chitosan/hydroxyapatite（CS/HA） composite with excellent mechanical properties was prepared via in situ precipitation.In this method,the calcium and phosphorus precursors were firstly added into the chitosan solution,dispersed homogeneously.Then the solution was precipitated in 6%（by mass） NaOH solution.The precipitated hydrogel rods were then cross-linked in sodium tripolyphosphate-trisodium phosphate（TPP-TSP） solution.The properties of the composites were characterized by means of TEM,FTIR,XRD and the three point bending test.Additionally,burn-out tests were also carried out to determine the dispersive uniformity of the two phases.The results showed that the synthesized HA was nanometer crystals and dispersed uniformly in chitosan matrix.The bending strength and modulus of CS/HA were 133 MPa and 6.8 GPa,respectively.Cell compatibility was studied by culturing pre-osteoblasts（MC3T3-E1） in extraction of the material,through MTT assay.Results of MTT assay indicated that the CS/HA material was good in biocompatibility with pre-osteoblasts.All of the results indicated that the CS/HA composite is a potential material as internal fixation of bone fracture.

关 键 词：壳聚糖 羟基磷灰石 原位沉析 内固定 细胞相容性 

分 类 号：R318.08[医药卫生—生物医学工程]