H5N1亚型禽流感病毒HA基因重组新城疫病毒活载体疫苗株组织嗜性的研究  

Studies on the Histotropism of Recombinant Newcastle Disease Virus LaSota Vaccine Strain Expressing HAmut HA Gene of H5 Highly Pathogenicity Avian Influenza Virus

作　　者：董俊斌[1] 步志高[2] 

机构地区：[1]内蒙古农业大学,内蒙古呼和浩特010018 [2]中国农业科学院哈尔滨兽医研究所/兽医生物技术国家重点实验室,黑龙江哈尔滨150001

出　　处：《江西农业大学学报》2010年第4期668-672,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家"973"计划资助项目(2005CB523200)

摘　　要：为了研究H5N1亚型禽流感病毒HA基因重组新城疫病毒活载体疫苗株[La Sota-HAmut(GD)]的组织嗜性,用重组疫苗[La Sota-HAmut(GD)]人工感染2日龄SPF鸡,接种后定期采集病鸡的各组织器官制备成石蜡切片,用建立的检测石蜡切片中[rLa Sota-HAmut(GD)]病毒的免疫酶组化染色技术,对人工感染[rLaSota-HAmut(GD)]鸡发病后病毒的组织器官亲嗜性和动态分布规律进行研究。[rLa Sota-HAmut(GD)]在胞浆内复制,主要亲嗜气管黏膜的上皮细胞和固有层腺体细胞,肝小叶间胆管上皮细胞。病毒出现的先后顺序为气管,肝脏,肺脏。结果表明:外源基因HAmut的插入对LaSota疫苗株的组织嗜性的影响很大。Vaccination was given by eyedrop and intranasally instillation with LaSota-HAmut（GD） （2 × 106 EID50 /chicken） to two-day-old SPF chicken,after 1,3,5,10,15,18 days the trachea,lungs,livers,kidneys,brains,spleen,proventriculus,duodenum were collected.The distribution of viral antigen in tissues was detected by using immunohistochemical（IHC） staining.As a resault,in the chickens of LaSota-HAmut（GD） vaccination,specilfic staining was localized in the trachea submucosa cells,hepatocytes,bronchus epithelial cells,alveolar nacrophages;the appeating sequence of viral antigens in the organs was tracheal,lungs,livers.These results showed that foreign gene HAmut inserted rLaSota had a little influence on histotropism of viral antigen.

关 键 词：rLa Sota-HAmut(GD) 组织嗜性 研究 

分 类 号：S852.65[农业科学—基础兽医学] S858.32[农业科学—兽医学]