烤烟打顶后不同部位烟叶碳氮代谢关键酶活性的动态变化及相关分析  

Dynamic Changes of Activities of Key Enzymes in Carbon and Nitrogen Metabolism in Flue-cured Tobacco of Different Positions after Topping and Correlation Analysis

作　　者：吕中显[1] 赵铭钦[1] 赵进恒[1] 张迪[1] 张学杰[1] 刘洪华[1] 

机构地区：[1]河南农业大学烟草学院,河南郑州450002

出　　处：《江西农业大学学报》2010年第4期700-704,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家烟草专卖局重大科技攻关项目(110200401004)

摘　　要：通过对不同部位烤烟烟叶打顶时以及打顶后10d、20d、30d的硝酸还原酶、淀粉酶和转化酶活性变化规律进行研究,结果表明:(1)打顶时到打顶后20d上部烟叶中硝酸还原酶活性高于中、下部烟叶,中、下部烟叶中硝酸还原酶活性从打顶时到打顶后10d迅速升高。(2)三个部位烟叶中淀粉酶活性总体都呈上升趋势,打顶时淀粉酶活性都很低并基本相同,中部和上部烟叶淀粉酶活性分别在打顶后10d和打顶后20d有一个峰值。(3)整个时期内上部烟叶中转化酶活性始终大于中、下部烟叶,中、下部烟叶中转化酶活性呈一直下降趋势,下部烟叶的下降速率大于中部烟叶。(4)上部烟叶中淀粉酶活性与硝酸还原酶、转化酶活性均呈显著负相关,中部烟叶和下部烟叶中淀粉酶活性与转化酶活性达显著负相关水平。The variations of activities of nitrate reductase,amylase and invertase in the flue-cured tobacco leaves in different positions at topping and 10 d,20 d,30 d after topping were studied.The results showed that： 1） From topping to 20 d after topping,the activity of nitrate reductase in the upper leaves was higher than that in the cutters and lower leaves.From topping to 10 d after topping,the activity of nitrate reductase in the cutters and lower leaves rose rapidly.2） The activity of amylase of tobacco leaves in the 3 positions decreased during the whole stage in general and was very low and similar to that at topping.There was a peak activity value of amylase in the cutters and upper leaves 10 d after topping and another 20 d after topping respectively.3） The activity of invertase in the upper leaves was higher than that in the cutters and the lower leaves during the whole stage.The activity of invertase in the cutters and lower leaves showed continuous declining trend and the declining speed in the lower leaves was larger than in the cutters.4） The activity of amylase was correlated significantly and negatively with the activity of nitrate reductase and invertase in the upper leaves.There was also an obvious negative correction between the activity of amylase and that of invertase.

关 键 词：烤烟 碳氮代谢 酶活性 动态变化 相关分析 

分 类 号：S752.01[农业科学—森林经理学]