NTG-LiCl复合诱变选育链霉菌702菌株  

Mutation Screening of Streptomyces 702 by NTG and LiCl

作　　者：涂璇[1] 周云[1] 叶荣华[1] 涂国全[1] 

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045

出　　处：《江西农业大学学报》2010年第4期808-812,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江西省科技支撑计划项目(2009BNA07200)

摘　　要：为筛选出产新农抗702的高产链霉菌702突变株。分别以链霉菌702菌株为试验材料和以庆大霉素为敏感抗生素,NTG-LiCl复合诱变链霉菌702菌株,获得抗庆大霉素突变株。NTG处理90min对菌株的致死率可达71.56%,抗药性突变率高达19.46%,获得的抗药性突变株经过摇瓶初筛和复筛,获得高产突变株13-18-52菌株,产抗新农抗702的摇瓶发酵单位达到1946μg/mL,比出发菌株发酵单位1416μg/mL提高了37.43%。采用抗药性致死突变标志的NTG-LiCl复合诱变筛选模型可以获得产新农抗702的链霉菌702高产菌株。Purpose： To screen out a Streptomyces 702 strain of high yield of New Ag-antibiotics 702.Methods： Gentamycin-resistance mutational labelling selection model with Streptomyces 702 strain ws used as the experimental material and Gentamycin as sensitive antibiotic to screening high-yield mutants.The Streptomyces 702 spores was treated by NTG-LiCl composite mutation.Results： The results showed that the gentamycin-resistance mutational frequency of the streptomyces 702 treated by NTG-LiCl with 90 min utes was up to 19.46% and the lethal rate was 71.56% .High New Ag-antibiotics 702-producing mutant 13-18-52 was isolated in the screening experiments,and its yield of New Ag-antibiotics 702 reached 1946μg/mL.which was 37.43% higher than that produced by its parent strain.Conclusion： The Streptomyces 702 strain of high New Ag-antibiotics 702 could be obtained by NTG-LiCl composite mutation and resistance-chemical mutational labelling selection model.

关 键 词：NTG LICL 复合诱变 链霉菌702 新农抗702 

分 类 号：S182[农业科学—农业基础科学]