高效液相色谱法测定狼疮灵颗粒中芍药苷的含量  

Determination of paeoniflorin in langchuangling granules by HPLC

作　　者：孙川[1] 冯俭[2] 李煌[2] 

机构地区：[1]成都中医药大学药学院,四川成都610072 [2]成都中医药大学附属医院,四川成都610072

出　　处：《广州医药》2010年第5期56-58,共3页Guangzhou Medical Journal

摘　　要：目的建立狼疮灵颗粒中芍药苷的高效液相色谱（HPLC）含量测定方法。方法采用HPLC法,色谱柱：Hypersil ODS-2（150 mm×4.6 mm,5.0μm）,流动相∶乙腈-0.4%的磷酸水溶液（10∶90）,检测波长∶230nm,流速：1.0ml/min,柱温：25℃。结果芍药苷在0.608－3.04μg范围内呈良好的线性关系（r=0.9999）,加样回收率97.77%,RSD为0.62%（n=5）。结论该方法快速可行、简捷准确、重现性好、结果可靠,可用于控制狼疮灵颗粒的质量。Objective To establish an accurate method for determination of Paeoniflorin in Langchuangling Granules.Methods Hypersil ODS-2（4.6mm×150mm,5.0μm） in an oven at 25℃ was need,with a mobile phase consisting of acetonitril-0.4% granolite treatment（10∶90）and UV detector at 230nm,the flow rate 1.0 ml/min.Results The linear range of Paeoniflorin was within 0.708～3.54μg（r=0.9999）.The average recovery was 97.77% and RSD was 0.62%（n=5）.Conclusion The method is simple and accurate.It can be used for the quality control of Langchuagnling Granules.

关 键 词：狼疮灵颗粒 芍药苷 高效液相色谱法 

分 类 号：R286[医药卫生—中药学]