光度法研究姜黄素与铁(Ⅲ)的络合反应  

Spectrophotometric study of curcumin's complex reaction with iron(Ⅲ)

作　　者：程忠洲[1] 屈英格[1] 李华[1] 司马小线 

机构地区：[1]西北大学分析科学研究所,陕西西安710069 [2]西安力邦制药有限公司,陕西西安710075

出　　处：《西北大学学报（自然科学版）》2010年第4期627-630,共4页Journal of Northwest University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(20675063)

摘　　要：目的研究姜黄素与铁(Ⅲ)的络合反应,并建立铁的测定方法。方法采用分光光度法。结果在10^(-5)～10^(-3)mol/L范围内,姜黄素有不同的吸收光谱;其与铁络合物的吸收波长由430nm红移至496 nm,呈增色反应。在pH 5.60,浓度为6.51×10^(-4)moL/L姜黄素液中,络合物为酒红色,λ_(max)=496 nm,ε=3.86×10~6L/mol·cm,铁(Ⅲ)的线性范围为0.20～2.00μg/mL。结论在较高浓度下自动聚合的姜黄素与铁(Ⅲ)反应更灵敏。在此基础上,建立的姜黄素测定铁的分光光度法操作简单,选择性较好,灵敏度高,用于自来水中铁(Ⅲ)的测定,结果满意。Aim To study the curcumin＇s complex reaction with Fe （Ⅲ） and develop a spectrophotometric method of determination of Iron （Ⅲ）. Methods Spectrophotometry was used. Results In the range of 10^ -5 - 10^-3 mol/ L, curcumin had different absorption spectrograms. The absorption wavelength of the curcumin-Fe （Ⅲ） complex was red shift from 430 nm to 496 nm and its absorbance increased. In the pH 5.6 solution containing 6. 51 ^ 10 ^-4 mol/L curcumin, the complex is claret-red and the molar absorptivity is 3.86 × 10^4 L/mol · cm at λmax = 496 rim. The calibration curve is linear in the range 0. 20 - 2. 00 μg/mL for Fe （Ⅲ）. Conclusion At the higher concentration, polymerized curcumin is more sensitive to react with Fe （Ⅲ）. Based on this, a simple, selective and sensitive spectrophotometric method has been developed and applied satisfactorily to determine Fe（ Ⅲ） in tap water.

关 键 词：姜黄素 铁 分光光度法 络合反应 

分 类 号：O657.32[理学—分析化学]