高效毛细管电泳法测定乳氟禾草灵的含量  

Determination of Lactofen by High Performance Capillary Electrophoresis

作　　者：阎峰[1] 关瑾[1] 陈星[1] 石爽[1] 傅业荣 刘晓霞[2] 

机构地区：[1]沈阳化工大学,沈阳110142 [2]东北大学,沈阳110004

出　　处：《农药》2010年第9期662-663,共2页Agrochemicals

基　　金：辽宁省教育厅高等学校科学研究基金项目(20060680);江苏长青农化股份有限公司企业博士后基金项目(0501057B)

摘　　要：建立了高效毛细管电泳法测定乳氟禾草灵的含量。使用有效长度为42cm、内径为50μm的毛细管,在背景电解质为50mmol/L硼砂-150mmol/L磷酸二氢钠(pH值9.5)、检测波长为281nm、分离电压为15kV的优化条件下,分析时间小于6min。乳氟禾草灵质量浓度在10～240mg/L内线性关系良好,相关系数为0.9997;方法的检测限(S/N为3)和定量限(S/N为10)分别为0.8、2.0mg/L;加标回收率为98.2%～99.0%,相对标准偏差为0.59%～2.6%。A high performance capillary electrophoresis （HPCE） method has been established for the analysis of lactofen. The separation was carried out on a fused-silica uncoated capillary column（42 cm×50 μm i.d.）. The analysis was completed in less than 6 min in 50 mmol/L borox-150 mmol/L sodium dihydrogen phosphate buffer（pH 9.5） at 15 kV of separation voltage and 281 nm of detection wavelength. A good linearity was obtained for lactofen in the range from 10 to 240 mg/L with correlation coefficient was 0.9997. The limits of detection（LOD） and quantification （LOQ） were 0.8 mg/L（S/N 3） and 2.0 mg/L（S/N 10）, respectively. The average recoveries were in the range of 98.2-99.0%, and relative standard deviations（RSDs） were between 0.59 and 2.6%.

关 键 词：毛细管电泳法 乳氟禾草灵 含量测定 

分 类 号：TQ457[化学工程—农药化工]