高血压病肝阳上亢证患者血清样品的核磁共振谱代谢组学研究  

A nuclear magnetic resonance spectroscopic metabonomics analysis for serum samples of hypertension patients with hyperactivity of liver-yang

作　　者：蒋海强[1] 马斌[2] 聂磊[2] 李运伦[3] 

机构地区：[1]山东中医药大学教学实验中心,山东济南250335 [2]山东大学药学院,山东济南250011 [3]山东中医药大学附属医院高血压中医临床研究基地,山东济南250011

出　　处：《药学实践杂志》2010年第4期258-261,共4页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金资助课题(30772865);教育部新世纪优秀人才支持计划项目(NCET-07-0522);山东省优秀中青年科学家奖励基金(2008BS03029);山东省2009-2010中医药科技发展计划项目(2009-018)

摘　　要：目的采用1HNMR结合主成分分析(PCA)方法研究高血压病肝阳上亢证患者和健康志愿者的血液成分谱差异,寻找可能的生物标记物,揭示高血压病肝阳上亢的证候本质。方法根据临床高血压病肝阳上亢证的诊断标准,选择典型病例,以1HNMR分析高血压病肝阳上亢证患者和健康志愿者的血液成分,使用PCA方法进行模式识别,寻找标记物并做出相关代谢途径的可能解释。结果PCA方法处理高血压病肝阳上亢证患者和健康志愿者的血清1HNMR谱数据显示,两组1HNMR谱数据可以在得分图实现分类,与健康志愿者作比较,患者机体相关代谢发生显著变化,体内部分氨基酸代谢和葡萄糖代谢明显异于健康志愿者。结论1HNMR结合PCA模式识别的代谢组学方法具有研究复杂条件下机体病理生理变化的优势,为理解高血压病肝阳上亢证的证侯本质和诊断该类病证提供了科学依据。Objective To apply 1HNMR spectroscopic analysis and principal components analysis（PCA） for investigating the difference of blood composition spectrum between hypertension patients with hyperactivity of liver-yang and healthy volunteers, to search possible biomarker, Reveal the nature of syndrome of hyperactivity of liver-yang. Methods Choose typical cases according to the clinic diagnostic criteria for liver-yang hyperactivity hypertension, and analysis the components in the blood of hypertension in the patients with syndrome of ascendant hyperactivity of liver-yang and healthy volunteers by 1HNMR. Track biomarkers and make the possible explanation of the relevant metabolic pathways with pattern recognition by the PCA method. Results The data from the analysis by PCA of the serum samples of hypertension in hypertension patients with syndrome of ascendant hyperactivity of liver-yang and healthy volunteers shows that： two sets of data can be classified in the score plot. There is significant differences existing be- tween the two groups in terms of relative metabolism of body, especially for some amino acid metabolism and Glucose metabolism. Conclusion The metabonomics method,1HNMR combined with PCA pattern recognition, has advantages in studying pathophysiological changes of the body under the complicated conditions. The study provided evidences for probing the essence of the hypertension of syndrome of ascendant hyperactivity of livcr-yang and researching the clinical diagnosis of such diseases.

关 键 词：代谢组学 核磁共振波谱 主成分分析 高血压 肝阳上亢 

分 类 号：R963[医药卫生—微生物与生化药学]