知母皂苷B-Ⅱ抗抑郁作用及其机制研究  

Study on the effect and mechanisms of timosaponin B-Ⅱ on antidepressant

作　　者：路明珠[1] 张治强 伊佳[1] 陈万生[1] 侯忠海[3] 李铁军[3] 

机构地区：[1]第二军医大学附属长征医院药剂科,上海200003 [2]解放军第321医院药材科,黑龙江白城137000 [3]第二军医大学药学院药理学教研室,上海200433

出　　处：《药学实践杂志》2010年第4期283-287,共5页Journal of Pharmaceutical Practice

摘　　要：目的研究知母皂苷B-Ⅱ抗抑郁的作用,并初步探讨其作用机制。方法采用小鼠悬尾实验、小鼠强迫游泳实验、开野实验;阿朴吗啡致小鼠刻板行为实验;抑制单胺(去甲肾上腺素NE和多巴胺DA)重摄取实验;育亨宾毒性增强实验;以及5-羟色胺酸(5-HTP)致甩头作用等动物模型来考察知母皂苷B-Ⅱ抗抑郁作用及其可能的机制。分别以小鼠不动时间、自主活动数、刻板行为评分、死亡率作为评价指标。结果在小鼠悬尾实验中,知母皂苷B-Ⅱ(100、150 mg/kg)能够明显缩短小鼠的不动时间;在小鼠强迫游泳实验中,知母皂苷B-Ⅱ(50、100、150 mg/kg)均能够明显缩短小鼠的不动时间;对开野实验中小鼠的自主活动次数无明显影响。在抑制NE和DA重摄取实验中,知母皂苷B-Ⅱ高剂量(150 mg/kg)显著增加多巴胺(DA)致小鼠死亡作用,但对去甲肾上腺素(NE)重摄取抑制作用不明显。阿朴吗啡致小鼠刻板运动实验显示,知母皂苷B-Ⅱ(100、150 mg/kg)对注射阿朴吗啡后的小鼠刻板运动有显著增强作用,差异具有显著意义(P<0.05);具有增加5-羟色胺酸(5-HTP)致甩头作用;知母皂苷B-Ⅱ不具有增加育亨宾毒性的作用。结论知母皂苷B-Ⅱ有抗抑郁活性,其作用机制可能与增强脑内5-HT、DA神经系统作用有关。Objective To study the effect and mechanisms of timosaponin B- 11 on depression. Methods The tail suspension test（TST）, forced swimming test（FST）, open field activity test （OFA）, APO-induced synergistic action test, inhibiting reuptake of monoamine neurotransmittcrs and monoamine oxidasc inhibitor（MAOI） test,yohimbine-induced lethality test and 5-HTP-induced head- twitch test were carried out to examine the antidepressant effect and mechanisms of timosaponin B- 11 in rats. Immobility duration in FST and TST,spontaneous activity frequency in OFA,the stereotyped behaviors scores in APO-induced synergistic action test,and mor- tality in the other tests were observed. Results Timosaponin B- II （100,150mg/kg） could effectively shorten the immobility time in TST and FST, but all dosages showed no significant effect on the spontaneous activity frequency in OFA. Timosaponin B-II （150 mg/ kg） significantly increased the rate of death of injecting dopamine, but had no obvious effect on the inhibiting reuptake of NE. Timosaponin B-II （100,150 mg/kg） enhanced the apomorphine-indueed stereotypy remarkably, which with statistically significant differences （P 〈 0.05） , and increased the head-twitch response evoked by 5-HTP, but had no effect on the yohimbine toxicity. Conclusion Timosaponin B- II has significant antidepressant effect which may be related to the potentiation of brain 5-HT nerve system and DA nerve system.

关 键 词：抗抑郁 知母皂苷 作用机制 

分 类 号：R965[医药卫生—药理学]