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作 者:王曾珍[1] 白史且[1,2] 张玉[2] 干友民[1]
机构地区:[1]四川农业大学草业科学系,四川雅安625014 [2]四川省草原科学研究院,四川成都611731
出 处:《西南农业学报》2010年第4期1238-1245,共8页Southwest China Journal of Agricultural Sciences
基 金:四川省应用基础项目(2008JY0010);四川省科技厅十一五牧草育种攻关(06SG023-001);国家牧草产业体系项目资助
摘 要:分别用60Co-γ射线、NaN3和航天诱变以不同剂量处理普那菊苣种子,对诱变处理后的幼苗进行了生理生化特性测定,结果表明:普那菊苣幼苗对60Co-γ射线、NaN3和航天诱变的刺激在生理生化特性上显示出不同程度的应激效应。其中在游离脯氨酸积累方面,辐射诱变幼苗的积累量大于航天诱变和化学诱变;而在可溶性蛋白含量上,航天诱变的效应大于化学诱变和辐射诱变;在可溶性糖含量上则是化学诱变的积累量最大,高于辐射诱变和航天诱变的刺激效应。The seeds of Puna Chicory were irraditiated by γ-ray,chemical by NaN3 and were spaced at different dose.The seedlings were measured by physiological and biochemical characteristics.The results indicated that 60Co-γ ray,NaN3 and space mutagenesis make Puna chicory seedlings showing different degrees of stimulation effects on physiological and biochemical characteristics.In the accumulation of free proline,radiation-induced accumulation of seedlings was greater than space mutagenesis and chemical mutagenesis;in the content of soluble protein,the space was greater than the effect of chemical mutagen and radiation-induced mutation;in the content of soluble sugar,chemical mutagenesis had the largest accumulation,which was higher than that of the radiation-induced mutagenesis and space.
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