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作 者:孔琼[1,2] 袁盛勇[3] 叶海燕[1] 刘振华[2] 王虹霞[1] 王德琴[1] 王云月[2]
机构地区:[1]红河学院生物系,云南蒙自661100 [2]云南农业大学植保学院,云南昆明650201 [3]红河学院农学系,云南蒙自661100
出 处:《西南农业学报》2010年第4期1380-1382,共3页Southwest China Journal of Agricultural Sciences
基 金:红河学院校级课题[XJ1Y0803];红河学院生物化学与分子生物学重点学科建设项目;红河学院生物科学重点专业建设项目[ZD200803]
摘 要:本实验从碳氮源、pH值、光周期及湿度对枇杷炭疽菌生长的影响进行研究。结果表明,该菌菌落生长的最佳碳源是麦芽糖,氮源是蛋白胨;促进产孢量增加的最佳碳源是麦芽糖,氮源是尿素。在pH值为3~10内,菌丝均能生长和产孢,菌丝扩展最适pH值为6,产孢量最大和分生孢子萌发率最高的pH值为5。全光照处理有利于菌落扩展和产孢量的增加,但抑制了分生孢子的萌发。分生孢子在饱和湿度中萌发率较高,相对湿度低于81%时不能萌发,且致死温度为50℃、10 min。The effects of carbon and nitrogen source,pH value,photoperiod,humidity on the colony growth,sporulation and conidial germination of Colletotrichum gloeosporioides Penz.were studied.The results showed,for the colony growth,the optimum carbon source was maltose,the optimum nitrogen source was peptone;About sporulation quantity,the optimum carbon source was maltose,and the optimum nitrogen source was urea.It grew between pH 3-10,and optimum pH value was 6 for colony growth,pH value of the largest quantity in sporulation and the highest of germination rate was 5.Illumination was propitious to the colony growth and sporulation,but it inhibited the conidial germination of the fungi.The germination rate of conidia was higher in saturated humidity than others,it could not germinate when the relative humidity below 81%.The lethal temperature of conidia was 50 ℃ for 10min.
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