濒危植物香果树愈伤组织经长期继代后的6种同工酶研究  

Studies on Six Kinds of Isozymes after Long-term Subculture of Emmenopterys henryi Oliv.

作　　者：杜景川[1] 景丹龙[2] 陈发菊[2] 李凤兰[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]三峡大学生物技术研究中心,湖北宜昌443002

出　　处：《安徽农业科学》2010年第19期10010-10014,共5页Journal of Anhui Agricultural Sciences

基　　金：国家林业局自然保护区研究中心项目(460-8101);国家林业局948项目(2006-4-73)

摘　　要：[目的]研究濒危植物香果树愈伤组织经长期继代后的6种同工酶酶谱的变化。[方法]采用用非变性聚丙烯凝胶电泳技术对长期继代后的香果树愈伤组织的酯酶(EST)、酸性磷酸酯酶(ACP)、ATP酶(ATPase)、淀粉酶(AMY)、超氧化物歧化酶(SOD)和过氧化物酶(POD)6种同工酶进行分析。[结果]香果树的胚性愈伤组织和非胚性愈伤组织在6种同工酶水平上均有差异,均可作为鉴别2者的依据。非胚性愈伤组织中的EST、ACP和POD的表达量明显高于胚性愈伤组织。褐化的非胚性愈伤组织与正常非胚性愈伤组织相比,在AMY、SOD和POD同工酶水平上无差别,而EST、ACP和ATPase同工酶含量减少;褐化的胚性愈伤组织与正常胚性愈伤组织相比,EST同工酶含量升高,其他5种同工酶含量降低。[结论]为研究香果树的长期组织培养过程中愈伤组织形态差异及褐化现象提供理论基础。[Objective]The aim was to study the changes of zymography in six kinds of isozymes after long-term subculture of Emmenopterys henryi Oliv..[Method]Non-denaturing polyacrylamide gel electrophoresis was used to analyze isozyme patterns such as esterase（EST）,acid phosphatase（ACP）,ATP enzyme（ATPase）,amylase（AMY）,superoxide dismutase（SOD）and peroxidase（POD）in long-term subculture callus of Emmenopterys henryi Oliv.[Result]The research showed that,there were differences among the six kinds of isozymes in embryogenic callus and non-embryogenic callus of Emmenopterys henryi Oliv.and both levels could be taken as the basis for identification,the EST,ACP,and POD of non-embryogenic callus were significantly higher than embryogenic callus.The browning of non-embryogenic callus was non-level in the AMY,SOD and POD isozymes when was compared with normal non-embryogenic callus,while the EST,ACP and ATPase isozymes decreased;When the browning of embryogenic callus was contrasted with normal embryogenic callus,EST isozyme increased and the other five kinds of enzymes decreased.[Conclusion]The study provided theoretical basis for research morphological difference and browning of long-term tissue culture of Emmenopterys henryi Oliv..

关 键 词：香果树 愈伤组织 长期继代 同工酶 褐化 

分 类 号：Q945.6[生物学—植物学]