AtNHX1提高烟草耐盐性的研究  

Study on Improving Salt Tolerance by Transformation of Tobacco with AtNHX1 Gene

作　　者：周树峰[1] 兰海[1] 郑名敏[1] 唐祈林[1] 

机构地区：[1]四川农业大学玉米研究所,四川雅安625014

出　　处：《安徽农业科学》2010年第19期10059-10060,10064,共3页Journal of Anhui Agricultural Sciences

基　　金：国家973项目(2007CB108907)

摘　　要：[目的]探讨利用AtNHX1基因提高烟草耐盐性的效果。[方法]利用农杆菌侵染的方法对烟草的叶盘进行遗传转化,将拟南芥的液泡膜Na+/H+逆向转运蛋白基因AtNHX1成功地转入了烟草中,对获得的28株潮霉素抗性转基因植株中的6株进行了Southern检测,并用200mmol/LNaCl对转基因植株进行盐分胁迫与耐盐性鉴定。[结果]分子检测表明,AtNHX1已经整合到烟草的基因组中,并得到了表达。在盐分胁迫下,各转基因株系具有较高的光合速率和PSⅡ活性,光合速度和Fv/Fm值均显著高于野生型对照,株系T1、T5的SOD酶活分别是野生型的1.8和2.1倍,各转基因株系GR活性也均显著高于野生型,而MDA含量则低于野生型。[结论]转At-NHX1基因的各株系的耐盐性较野生型对照有了较大程度的提高。[Objective] The study aimed to discuss the effect of improving the salt tolerance by transformation of tobacco with AtNHX1 gene.[Method] Arabidopsis vacuole Na＋/H＋ antiporter gene AtNHX1 was successfully introduced into the tobacco genome by Agrobacterium tumefaciens mediated transformation on the tobacco leaf.The southern examination was made on the 6 plants from the obtained 28 transgenic plants with the resistance to hygromycin.The transgenic plants were made for the salt stress with 200 mmol/L NaCl and the salt tolerance identification.[Result] Molecular test showed that AtNHX1 had been integrated into the tobacco genome and was expressed properly.Under the salt stress,each transgenic plant line had higher photosynthetic rate and PSII activity,with the photosynthetic rate and Fv/Fm value being higher than the wild type CK;the SOD activity in the plant lines T1 and T5 were 1.8 and 2.1 times of that in the wild type;the GR activity in each transgenic plant line was also obviously higher than that the wild type.While their MDA content was lower than the wild type.[Conclusion] The salt tolerance of each transgenic plant line introduced AtNHX1 gene had greatly increase compared with the wild type CK

关 键 词：烟草 ATNHX1 耐盐性 遗传转化 

分 类 号：S572[农业科学—烟草工业]