650nm激光和艾灸预处理对内脏牵拉痛大鼠肠神经系统和延髓内脏带的影响  

Effects of 650 nm laser and moxibustion pretreatment on enteric nervous system and medullary visceral zone in rats with visceral traction pain

作　　者：杨华元[1] 郭婷婷[1] 马忆南[1] 刘堂义[1] 高明[1] 

机构地区：[1]上海中医药大学针灸推拿学院,上海201203

出　　处：《中国针灸》2010年第9期745-751,共7页Chinese Acupuncture & Moxibustion

摘　　要：目的:探讨650nm激光和艾灸预处理对内脏牵拉痛大鼠的作用及作用机制。方法:40只雄性SD大鼠随机分为4组:假手术组(A组)、内脏牵拉痛组(B组)、650nm激光预处理组(C组)、艾灸预处理组(D组),A组仅做假手术,B组做内脏牵拉痛模型,A、B两组均不做其他处理;C组采用650nm激光照射"足三里"预处理后即刻做内脏牵拉痛模型,D组采用艾灸"足三里"预处理后即刻做内脏牵拉痛模型。检测疼痛积分、收缩压的变化,并运用生化比色、放射免疫法、免疫组化法,检测乙酰胆碱酯酶(AChE)活性、P物质(SP)、亮氨酸-脑啡肽(LEK)的含量和c-Fos蛋白及胶原纤维酸性蛋白(GFAP)阳性指数。结果:B组与A相比较,疼痛积分、收缩压、AChE活性、SP阳性表达物、c-Fos蛋白、GFAP阳性指数均升高,差异均有统计学意义(均P<0.05);与B组比较,C组和D组疼痛积分、AChE活性、SP阳性表达物、c-Fos蛋白、GFAP阳性指数均降低,差异均有统计学意义(均P<0.05);C组与B组相比,LEK含量升高,收缩压降低,差异均有统计学意义(均P<0.05)。结论:650nm激光和艾灸预处理方法均对内脏牵拉痛有抑制作用,其作用机制可能与减低AChE活性、SP含量,提高LEK的活性,下调c-Fos蛋白和GFAP的表达有关。Objective To explore effects of 650 nm laser and moxibustion pretreatment on visceral traction pain （VTP） and its mechanism. Methods Forty male SD rats were randomly devided into a sham operation group （group A）, a VTP group （group B）, a 650 nm laser pretreatment group （group C）, a moxibustion pretreatment group （group D）. Rats in group A and group B were not treated except sham operation or VTP model. In group C and D, the VTP models were produced immediate after 650 nm laser irradiation or moxibustion at ＂Zusanli＂ （ST 36）, respectively. The changes of pain score and systolic pressure were investigated and the activity of ACHE, the content of SP and leu-enkephaline （LEK）, and the positive index of c-Fos protein and glial fibrillary acidic protein （GFAP） were detected by biochemistry, radio-immunity method and immunohistochemistry, respectively. Results Compared with group A, the pain score, systolic pressure, the activity of ACHE, the content of SP, and the positive index of c-Fos protein and GFAP of group B increased significantly （all P〈0.05） ; compared with group B, the pain score, AChE activity, the content of SP and the positive index of c-Fos protein and GFAP of both group C and group D decreased significantly （all P〈0.05）; compared with group B, the content of LEK increased and systolic pressure decreased significantly in group C （both P〈0. 05）. Conclusion Both 650 nm laser and moxibustion pretreatment can inhibit VTP and the mechanism may be related to reducing the activity of AChE and the content of SP, and increasing the activity of LEK and decreasing the expression of c-Fos protein and GFAP.

关 键 词：激光预处理 艾灸预处理 内脏牵拉痛 肠神经系统 延髓内脏带 

分 类 号：R245[医药卫生—针灸推拿学]