艾灸“大椎”对荷瘤小鼠脑皮质白介素(IL)-1β、IL-2、IL-6 mRNA及蛋白表达的影响  

Effects of Moxibustion on the Expression of IL-1β,IL-2,IL-6 mRNA and Protein in the Cerebral Cortex in Tumor-bearing Mice

作　　者：裴建[1] 魏海[2] 刘志丹[1] 于颍梅[1] 倪灿荣[3] 吴焕淦[2] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032 [2]上海市针灸经络研究所,上海200032 [3]第二军医大学附属长海医院,上海200433

出　　处：《针刺研究》2010年第4期243-249,共7页Acupuncture Research

基　　金：国家自然科学基金资助项目(No.39900196);上海市科委中药现代化项目(No.04DZ19838);上海市重点学科建设项目(No.S30304)

摘　　要：目的:观察艾灸"大椎"治疗后荷瘤小鼠脑皮质白介素(IL)-1β、IL-2、IL-6 mRNA及蛋白表达的变化,探讨艾灸调节肿瘤免疫抑制状态的机制。方法:H22荷瘤小鼠随机分为:正常组、荷瘤组、艾灸组、非经穴组,每组10只。采用艾灸"大椎"治疗,原位杂交法检测脑皮质IL-1β、IL-2、IL-6 mRNA表达,免疫组化法检测脑皮质IL-1β、IL-2、IL-6蛋白表达。结果:与正常组比较,荷瘤组小鼠脑皮质中IL-1β、IL-2的mRNA和蛋白表达均显著下降,IL-6 mRNA和蛋白质表达显著上升(P<0.05,P<0.01);与荷瘤组比较,艾灸组小鼠脑皮质中IL-1β、IL-2 mRNA和蛋白表达显著上升,IL-6 mRNA和蛋白则显著下降(P<0.05)。结论:艾灸治疗能上调荷瘤小鼠脑皮质IL-1β、IL-2 mRNA和蛋白表达,下调IL-6 mRNA和蛋白表达。艾灸调节荷瘤小鼠机体免疫抑制状态可能与调节脑皮质IL-1β、IL-2、IL-6mRNA和蛋白表达有关。Objective To investigate the effect of moxibustion on the expression of IL-1β, IL-2 and IL-6 proteins and mRNA in the cerebral cortex in tumor-bearing mice so as to study its mechanism underlying immunomodulation. Methods Forty Balb/c mice were randomly divided into control, tumor-bearing, non-acupoint moxibustion （N-AM）and acupoint-moxibustion （AM） groups （n = 10/group）. Moxibustion was applied to ＂Dazhui＂ （GV 14）, once every other day for 6 times. The expression of IL-1β mRNA, IL-2 mRNA and IL-6 mRNA was detected by in situ hybridization,and the immunoactivity of IL-1β, IL-6 and IL-2 determined by immunohistochemistry. Results Compared to the control group,the expression levels of IL-1β mRNA and IL-2 mRNA, IL-1β and IL-2 in the cerebral cortex of the tumor-bearing group were down-regulated significantly （P〈0.05, P〈0. 01 ）, while those of IL-6 mRNA and IL-6 up-regulated significantly （P〈0.05）. Compared to the tumor-bearing group, the expression of IL-1β mRNA and IL-2 mRNA, IL-1β and IL-2 in the cerebral cortex in AM group were increased considerably （ P〈0.05, P〈0.01 ） ; while cortical IL-6 immunoactivity in N-AM group was decreased significantly （ P〈0. 05）, and IL-6 mRNA had no significant change in N-AM group （P〉0.05）. Comparison between AM and N-AM groups showed that the expression levels of cortical IL-1β mRNA and IL-2 mRNA, and IL-1β and IL-2 proteins of the former group were obviously higher than those of the later group （P〈0. 05, P〈0. 01 ）, while the immunoactivity of cortical IL-6 of AM group was significantly lower than that of N-AM group （P〈0. 05）. No significant difference between AM and N-AM groups in the expression of IL-6 mRNA （P〉0.05）. Conclusion Moxibustion treatment can up-regulate the expression of cortical IL-1β mRNA, IL-2 mRNA, IL-1β and IL-2 proteins, and down-regulate the expression of IL-6 mRNA and IL-6 in tumor-bearing mice, which may contribute to its effect in improving the immunosuppressing state under

关 键 词：艾灸荷瘤小鼠免疫抑制 白细胞介素基因表达蛋白表达 

分 类 号：R245.81[医药卫生—针灸推拿学]